Is it iPSC colony in my reprogramming culture or not?

More Bintang Tedjoabagaskara's questions See All

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Is this yeast contamination in my stem cell culture?

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Can you simulate microchannel flow in a mini-channel using scaling through the dimensionless parameters?

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If I have three years utility bills data, which one that should I use to calibrate my energy model results from eQUEST?

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How could high concentration of plasmid DNA (140 ng/microliter according to Nanodrop measurements) show very narrow band in visualization of the gel?

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Is it iPSC or not?

We tried to reprogram MSC. After 29 days post-electroporation of episomal vector (carrying Yamanaka Factors), we found this on our culture (see pictures). Is it iPSC? we planned to terminate it if...

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Clumping colony on my Reprogramming Culture, is it by any chance iPSC colony?

7 days ago I transfected my MSC with episomes carrying reprogramming factors and today I found this structure (see the pictures) which seems like it consists of small cells (?), do you guys think...

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2mm vs 4mm electroporation cuvette

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What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

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Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

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My rosetta strain has chloramp resistance and i am transforming my plasmid with amp resistance but not getting colonies on chloramp/amp LB plates?

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Has anyone calculated the effective concentration at 50% (EC50) value of different fungicides for the fungus Tilletia horrida?

How can we measure their colony diameter?

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Regarding solubility of Dorsomorphin in DMSO ?

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I have constucted a plasmid vector from puc19 with no antibiotic and lacz gene. The problem is screening for transformed cells. Any suggestions?

Get too many colonies, which are mostly non-transformants when tested by colony PCR

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I am not getting proper colonies while ligating one of fragments in my vector the colonies seems to be normal but in light it seems lysed any reason?

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Are these iPSC colonies?

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Can someone provide any tips for cell quantification on Fiji, ImageJ?

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Cloning restrction digestion needed before sequencing?

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04 July 2024 7,977 2 View

John Schloendorn

Ahh no, that's at best a large piece of debris, and at worst a mold

Justyna Augustyniak

No.

Christopher Lovejoy

Afternoon,

I concur, this is likely debris, or potentially fungus.

I would keep a close eye on this, potentially throwing this plate away to be sure.

iPSC colonies should be round and the cells close together.

Chris

Chuck A Arize

Chapter Human Pluripotent Stem Cells (iPSC) Generation, Culture, and...