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Questions related from Bintang Tedjoabagaskara
Mycoplasma is not visible under the microscope, is there any signs especially in iPSC culture? My iPSC seems to have great morphology, the medium is transparent still, and no moving rod shaped...
20 December 2024 6,416 1 View
I stored my fixed cells ( fixed with pfa) in FACS buffer (2% FBS in PBS) at minus 20 celcius, would it be okay?
11 January 2023 1,840 3 View
I saw this colony which looks like cells that probably growing upward, is it by any chance an iPSC colony? I know iPSC colony normally would not looks like this, but has anyone encounter...
27 December 2022 4,082 4 View
I tried to reprogram MSC to iPSC and found my culture cloudy this morning, just want to ask whether this a contamination or not. thanks
14 December 2022 8,633 4 View
7 days ago I transfected my MSC with episomes carrying reprogramming factors and today I found this structure (see the pictures) which seems like it consists of small cells (?), do you guys think...
01 January 1970 4,384 1 View
We tried to reprogram MSC. After 29 days post-electroporation of episomal vector (carrying Yamanaka Factors), we found this on our culture (see pictures). Is it iPSC? we planned to terminate it if...
01 January 1970 9,034 5 View
Hello experts in genetic modification, according to your experiences, do 2 mm and 4 mm electroporation cuvette have any differences in performance? or they just different in volume thank you
01 January 1970 8,070 0 View
