Can I quickly study biofilm in bacterial broth without re-incubating after 0, 24, 48, and 72 hours of the experiment?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Trying to make optimization for Mixer on different pdk ?

i make a design topology of mixer For my graduation project working at 28 Ghz on cadence with UMC pdk and get this result S(2,1) 3.5db but when trying to make same topology with another pdk on...

09 June 2024 9,729 3 View

Is it good choice to use the SARC/J auxiliary basis set with def2-SVPD basis set without including any relativistic effects like ZORA or whatever?

I’m currently utilizing the ORCA software for my computations, applying the PBE0 functional combined with the def2-SVPD basis set and incorporating the RIJCOSX approximation. For the auxiliary...

07 June 2024 6,352 0 View

Do the methods of calculating the absorption coefficient (Alpha) from absorbance differ between powder samples and thin films?how is it calculated ?

When using the absorption spectrum to determine the absorption coefficient (Alpha), do the calculation methods differ between powder samples and thin films? If there are differences, what are the...

07 June 2024 8,797 2 View

How do quick initial-test to select antibiotic-producing fungal strains?

We have dozens of pure fungal strains recovered from the soil and need to know the extent of their ability to produce antibiotics. Still, the problem is the spread of fungal mycelia on the plate...

09 May 2024 9,467 2 View

Does anyone have detailed methods and instructions on how to count the numbers of intraepidermal nerve fibers (IENFs)/mm using Fiji - ImgaeJ?

Counting the numbers of intraepidermal nerve fibers

05 May 2024 3,296 1 View

Effects of Aeromonas caviae and Aeromonas veronii on Freshwater Fish?

28 April 2024 9,191 1 View

Convert solid organic powder to nanoparticle?

i synthesized schiff base with bulk size., any suitable method to convert it in nanosize with full regards

22 April 2024 1,423 2 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Why does my starch-curcumin bioplastic look bubbly?

My colleague and I started research project about bioplastic made out of starch-curcumin composite. However, we are currently having trouble regarding the produced film. We have degassed the...

01 July 2024 9,419 0 View

Help with in vitro testing of candida biofilm susceptibility?

Hi all, Would anyone have any suggestions for articles and protocols for performing microdilution tests on candida biofilm? Do you recommend diluting the drug in the medium and adding it to the...

19 June 2024 4,420 3 View

Can we call N-acyl homoserine lactone (N-AHL), a biomarker for the formation of biofilms?

Is there scientific evidence that proves that the presence of N-acyl homoserine lactone can account for the bacterial biofilm population?..

13 June 2024 3,776 2 View

What is the best method for diagnosing biofilm formation on liquefaction tubes used in poultry farming fields?

biofilm

31 May 2024 1,969 4 View

Can 100% alcohol (95% EtOH, 5% methanol/isopropyl alcohol) be used for bacterial SEM dehydration?

I am preparing a protocol for SEM of a biofilm, but do not have easy access to 100% ethanol for the dehydration steps. Can 100% ethanol be substituted for 100% alcohol (95% EtOH, 5%...

07 May 2024 1,515 3 View

Using metaproteomics, I plan to find out which specific proteins and enzymes are causing the severe corrosion on the metal surface. It is possible ?

I need some clarification about metaproteomics. In the wastewater (or) biofilm samples, I plan to find out which specific proteins and enzymes are causing the severe corrosion on the metal surface...

21 April 2024 5,315 2 View

Hi everyone I need to material and methods to biofilm effect of lactic acid bacteria against pathogenic bacteria?

Lactobacillus planetarium and Pediococcus pentosaceus against foodborne pathogens.

18 April 2024 8,127 0 View

Which are the best softwares for data analysis and graphs for the quantitative study of microbial biofilms?

Based on your expertise, which softwares are the best for the data analysis and graphs for the quantitative study of microbial biofilms?, pros & cons?

08 April 2024 8,304 1 View

Why is my Synechococcus sp. cyanobacteria losing its color and producing mats of biofilm, I suspect it might be either biofilm or some form of EPS ?

I use BG 11+ Media but with an alternative trace metal mix.

17 March 2024 9,253 3 View