Mohammed Tabrez Banegar

4 Questions 8 Answers 0 Followers

Questions related from Mohammed Tabrez Banegar

Why is there a yellow coloration in the sample on performing Zeta analysis of a protein sample?

On performing Zeta potential analysis of a protein in a buffer system (50mM Tris, 250mM NaCl, 6M Urea, 15mM BME, 1mM EDTA, 1mM PMSF). A canary yellow colouration was observed what could have led...

22 August 2024 8,406 2 View

On culturing cells with DMEM High Glu. The pH of medium decreases in few hours; even if the confluency of cells is <20-30%. Need troubleshooting idea?

I’m culturing NIH3T3 cells with DMEM high Glucose. I’ve observed that the media’s pH drops down significantly (yellowing of medium) in less than 24h, subjecting the cells to stress. Thus the cells...

19 August 2024 980 2 View

How do I calculate E.C50 or E.D50 by performing Haemeagglutination assay?

I intend to design a study which would require Haemeagglutination assay for a growth factor (g.f) and have referred to few website which have mentioned the E.D 50 value for that g.f. but I am...

30 May 2024 5,368 6 View

Protocol of SP2/O toxicity assay

Animal tissue culture media formulations in their datasheets mention SP2/O toxicity assay as one of their parameters. However, I am unable to find the protocol in literature. Any help would be...

01 January 1970 9,539 2 View