Why is my Synechococcus sp. cyanobacteria losing its color and producing mats of biofilm, I suspect it might be either biofilm or some form of EPS ?

More Alina Heiling's questions See All

How is it possible to model a protein that consists of several subunits using a Modeller?

Good day, I am trying to model the GABA A receptor, with different subunits. I selected the templates with the highest percent of identity, but as a result of the modeling I got a protein with...

02 June 2024 5,489 2 View

How to make a proper topology file for CNT-aminoacids water solution?

My aim is to simulate cnt with aminoacids(histidine) in water solution. I`ve made two separate topology files(15 molecules of histidine with pdb2gmx command and cnt topology with x2top) then merge...

20 April 2024 1,760 0 View

My Strep-tagged protein doesn`t bind IBA Tactin XT 4Flow spin columns. Could you please advice how to solve the problem with bindning to resin?

My protein is UFM1 tagged with Twin-Strep tag. I double-checked and my tag is not cleaved off or hidden. I made purification with IBA kit (Tactin XT 4Flow high capacity spin kit) with subsequent...

26 November 2023 4,087 3 View

With Vina, why did I get an output file with a different ligand location than the coordinate I specified in the configuration file?

Good day, I created a grid using the Autodock tools and then using those coordinates and size I created a configuration file for Vina. But in the results, the location of the ligand do not match...

12 November 2023 2,831 2 View

Dear colleagues, Do we have research/researches demonstrating exercising related changes in children?

I am working in Paediatric Outpatient Physiotherapy department with children with Lower Limb problems. As a part of our help to children we are running 6 weeks strengthening groups. The question...

19 September 2023 2,569 0 View

Should I order necessarily azide free primary antibodies for IHC, if I have HRP conjugated secondary antibodies?

I was wondering if the amount of sodium azide in primary antibodies for IHC can affect HRP activity in secondary antibodies, as I wash slides several times between the primary and the secondary...

12 September 2023 9,463 1 View

Does anybody have a good working protocol for IHC (tryptase) in mast cells?

I am a medical doctor and I do not have any experience in IHC, only toluidine blue and HE staining. Can you please share a protocol with me? Thank you in advance

12 September 2023 7,596 2 View

How to interpret the following RMSDS?

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21 August 2023 7,117 1 View

What to do when the protein structure has high RMSD due to loops?

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06 August 2023 7,344 3 View

Heat not flowing / transferring in Abaqus Fluid - Structure Co-simulation Conjugate Analysis. Can someone please help?

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25 March 2023 7,230 2 View

Is skin yellowness an numerical or ordinal variable?

I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...

11 August 2024 4,793 1 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 192 4 View

Hello researchers, how can one get the optical density of a filamentous algae?

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30 July 2024 5,985 2 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

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Why applying a saved template of a bar chart in a different/new one doesn't take effect in SPSS?

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29 July 2024 1,981 1 View

How to estimate sample size for GWAS of continuous and discrete traits? What are the pre-requisites?

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28 July 2024 286 0 View

Can anyone help me explain my problem?

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26 July 2024 2,134 0 View

Aqua regia not heating up?

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25 July 2024 4,390 0 View

What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS?

What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS? Hi I am trying to make the purification (by TCA...

22 July 2024 5,334 6 View

Jakub Grzesiak

This might be connected to heavy metal poisoning. What trace metal mix are you using and how much you're adding to the medium?

Alina Heiling

FeSO4 40g/l

MnSO4 10g/l

AlCl3 10g/l

CoCl2 7,3 g/l

ZNSO4 2,0 g/l

NA2MoO4 2,0 g/l

CuCl2 1,0 g/l

H3BO3 0,5 g/L This is the stock solution. We use 1ml/L for the BG11+ media. We already had this in mind. Its not the recommended composition.@Jakub Grzesiak

It seems very concentrated. Try adding it in the range of 100-200uL/L.