Is there any other free esy software than the Neurolucida?

Dear I have the pleasure to ask about any free download soft ware like neurolucida to extract borders and cells shape from the histological images of brain sections like that . as an examples in...

05 June 2019 3,748 6 View

RASP software for MCMC?

01 February 2016 9,701 1 View

How can I preserve brain tissue for future IF and histological analyses?

I want to preserve brain tissues for IF and histology later, for a long time. Put into consideration that I will transfer them to another country with 2 days traveling. I want to keep all...

09 October 2014 804 10 View

How can I avoid high mortality of rotenone-induced PD?

I used 1.5mg/kg dissolved in DMSO/PEG 1:1 and I have high mortality intraperitoneal injection.

09 October 2014 9,844 1 View

What I have to do in parkinson's disease model?

in the induction I dissolve rotenone in DMSO and later dilute it with saline to arrive 30% dilution of DMSO as soon as saline added rotenone precipitate.

06 July 2014 8,513 2 View

Is there any other factor for the induction of Parkinsonism with rotenone? I inject it 1.5 1:1 PEG and DMSO, but result in a high mortality rate.

problem in the injection of the rotenone to induce PD. I inject it 1.5mg/kg 1:1 PEG and DMSO, which results in a high mortality rate.

02 March 2014 2,937 3 View

If I want to calculate the MRCA for Bufo arabicus using RelTime, what calibrated taxa do I have to select?

And what is the divergence time I have to use? Thanks in advance.

01 February 2014 9,429 1 View

How can I estimate the sequence of the SiRNA that will be used?

I want to prevent the expression of 11 amino acid in the peptide chain using siRNA,

01 February 2014 3,570 2 View

How can I test the best model JC or HKY G+I model? On what basis can I say that one is better than the other?

How can I explain this?

31 December 2013 4,246 15 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View