dear all
I am looking for cell type ready expressing toxic beta amyloid.
Dear I have the pleasure to ask about any free download soft ware like neurolucida to extract borders and cells shape from the histological images of brain sections like that . as an examples in...
05 June 2019 3,862 6 View
Deal all, Please I want to use the RASP software for my samples to construct divergence time among my samples, different samples with different species of Bufoidea. I dont know what is the...
01 February 2016 9,820 1 View
I want to preserve brain tissues for IF and histology later, for a long time. Put into consideration that I will transfer them to another country with 2 days traveling. I want to keep all...
09 October 2014 921 10 View
I used 1.5mg/kg dissolved in DMSO/PEG 1:1 and I have high mortality intraperitoneal injection.
09 October 2014 9,950 1 View
in the induction I dissolve rotenone in DMSO and later dilute it with saline to arrive 30% dilution of DMSO as soon as saline added rotenone precipitate.
06 July 2014 8,627 2 View
problem in the injection of the rotenone to induce PD. I inject it 1.5mg/kg 1:1 PEG and DMSO, which results in a high mortality rate.
02 March 2014 3,033 3 View
And what is the divergence time I have to use? Thanks in advance.
01 February 2014 9,550 1 View
I want to prevent the expression of 11 amino acid in the peptide chain using siRNA,
01 February 2014 3,689 2 View
How can I explain this?
31 December 2013 4,370 15 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
