02 February 2016 1 10K Report

Deal all, 

Please I want to use the RASP software for my samples to construct divergence time among my samples, different samples with different species of Bufoidea.

I dont know what is the calibrator which I have to use for my samples as my sequences were sequenced for d loop and 12s of mtDNA and unfortunately, there is no data published for time calibration using these two genes. According this problem. i had to use the RASP as it using the substitution rate but I tried alot to use its tutorial but I could not.

Please I need help

Hani

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