What I have to do in parkinson's disease model?

More Hani Hafez's questions See All

Is there any other free esy software than the Neurolucida?

Dear I have the pleasure to ask about any free download soft ware like neurolucida to extract borders and cells shape from the histological images of brain sections like that . as an examples in...

05 June 2019 3,862 6 View

Can I find cell lines expressing beta 42?

dear all I am looking for cell type ready expressing toxic beta amyloid.

08 September 2017 3,029 0 View

RASP software for MCMC?

Deal all, Please I want to use the RASP software for my samples to construct divergence time among my samples, different samples with different species of Bufoidea. I dont know what is the...

01 February 2016 9,820 1 View

How can I preserve brain tissue for future IF and histological analyses?

I want to preserve brain tissues for IF and histology later, for a long time. Put into consideration that I will transfer them to another country with 2 days traveling. I want to keep all...

09 October 2014 921 10 View

How can I avoid high mortality of rotenone-induced PD?

I used 1.5mg/kg dissolved in DMSO/PEG 1:1 and I have high mortality intraperitoneal injection.

09 October 2014 9,950 1 View

Is there any other factor for the induction of Parkinsonism with rotenone? I inject it 1.5 1:1 PEG and DMSO, but result in a high mortality rate.

problem in the injection of the rotenone to induce PD. I inject it 1.5mg/kg 1:1 PEG and DMSO, which results in a high mortality rate.

02 March 2014 3,033 3 View

If I want to calculate the MRCA for Bufo arabicus using RelTime, what calibrated taxa do I have to select?

And what is the divergence time I have to use? Thanks in advance.

01 February 2014 9,550 1 View

How can I estimate the sequence of the SiRNA that will be used?

I want to prevent the expression of 11 amino acid in the peptide chain using siRNA,

01 February 2014 3,689 2 View

How can I test the best model JC or HKY G+I model? On what basis can I say that one is better than the other?

How can I explain this?

31 December 2013 4,370 15 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

In passive sampling, can polymer-lipid partition coefficients be applied independent of environmental salinity?

For example, if low-density polyethylene (LDPE) is equilibrated with PCB contaminated marine and freshwater sediments, can LDPE-lipid partition coefficients be used to obtain equilibrium...

28 July 2024 263 1 View

How to convert water conductivity in mS/cm to Salinity and TDS?

In order to test different water parameters I need to know how to convert conductivity to Salinity and TDS in ppm or ppt. Give me the reference please.

27 July 2024 2,414 2 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

Kraft lignin and MMA(Methyl meth acrylate) free radical polymerization for synthesis of thermoplastics?

I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...

15 July 2024 9,827 1 View

Regarding solubility of Dorsomorphin in DMSO ?

I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...

11 July 2024 4,832 0 View

How can ongoing education and team-basedsimulations improve the effective use of normalsaline in clinical practice?

Overview of Normal Saline in Fluid Therapy: Mechanisms, and ... How can ongoing education and team-basedsimulations improve the effective use of normalsaline in clinical practice?

27 June 2024 3,972 1 View

What roles do different healthcare professionals(physicians, nurses, pharmacists) play in themanagement of normal saline therapy?

Overview of Normal Saline in Fluid Therapy: Mechanisms, and ... What roles do different healthcare professionals(physicians, nurses, pharmacists) play in themanagement of normal saline therapy?

27 June 2024 848 1 View

Nicola Simola

Hi, as per my experience, rotenone is vey hard to dissolve. When I worked with rotenone, I used to dissolve it in PEG and to sonicate it for at least 2h. Also, make sure to work under dark conditions; rotenone is very much light sensitive.

Hani Hafez

did you dissolved it in PEG only

or with DMSO 1:1. I tried to dissolve rotenone in DMSO alone with concentration of 30% to avoid any mortality sides after injection.