Is the amplification curve affected when I use RT-step on cDNA in real time one step RT-PCR?

While editing the cycling protocol for One Step real time RT-PCR, If used RT step (while sample used is cDNA). Will this affect the amplification as the temperature of RT step is 55 C (not...

03 April 2019 1,817 2 View

What are principles of Real Time PCR?

i need to know: Principles Protocol The best kits available with high efficiency results How to read and interpret the results?

31 December 2018 2,234 0 View

How can response to reviewer comment to remove a confused point or to prove a finding?

What the best way in Discussion section to remove a confusion on bacterial pathogen which has never recorded in the certain area and by PCR gave the same product size for a bacterium of our study...

04 May 2018 5,231 4 View

How can I manage large DNA sequences in DNA alignment?

During Blastin search, I found many sequences with Large sequence. (about 2105803 bp ). I faced a problem in adding them to DNA Alignment and in each time a message appeared "Warning: about to...

02 March 2018 2,076 7 View

Is there a site helps me to draw a map for use in epidemiological study to illustrate the geographical distribution of the disease?

I am working on epidemiological study, which aimed to study risk factors of infectious diseases distribution, spread and re-emerge in endemic areas. For this I am asking how can I draw a map...

02 March 2017 9,751 0 View

What are advantages studying the percentage of homology in protein sequences?

I have a revised manuscript sent for publication and the reviewer had asked from me insert the percentage of homology of protein sequences then discuss, however, I discussed the DNA sequences...

03 April 2016 3,575 6 View

Does anyone have experience with LAMP protocol instead of PCR assay?

I mean what are the principles of LAMP and How can I prepare my Lab for it ?

03 April 2016 3,546 2 View

What are the advantages of using amino-acid sequences in comparison to using nucleotide sequences for phylogenetic analysis?

I need know how can I use and interpret the amino acid sequences for phylogeny for the same homologous region

02 March 2016 7,205 16 View

I need a teaching video for Glanders and Strangles in equine describing epidemiology, clinical signs, diagnosis and control?

02 March 2015 5,882 3 View

Does anyone have experience with the Kappa test?

08 September 2014 6,734 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View