Mahmoud Mohy Elhaig

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Questions related from Mahmoud Mohy Elhaig

Is the amplification curve affected when I use RT-step on cDNA in real time one step RT-PCR?

While editing the cycling protocol for One Step real time RT-PCR, If used RT step (while sample used is cDNA). Will this affect the amplification as the temperature of RT step is 55 C (not...

04 April 2019 1,919 2 View

What are principles of Real Time PCR?

i need to know: Principles Protocol The best kits available with high efficiency results How to read and interpret the results?

01 January 2019 2,329 0 View

How can response to reviewer comment to remove a confused point or to prove a finding?

What the best way in Discussion section to remove a confusion on bacterial pathogen which has never recorded in the certain area and by PCR gave the same product size for a bacterium of our study...

05 May 2018 5,323 4 View

How can I manage large DNA sequences in DNA alignment?

During Blastin search, I found many sequences with Large sequence. (about 2105803 bp ). I faced a problem in adding them to DNA Alignment and in each time a message appeared "Warning: about to...

03 March 2018 2,180 7 View

Is there a site helps me to draw a map for use in epidemiological study to illustrate the geographical distribution of the disease?

I am working on epidemiological study, which aimed to study risk factors of infectious diseases distribution, spread and re-emerge in endemic areas. For this I am asking how can I draw a map...

03 March 2017 9,853 0 View

What are advantages studying the percentage of homology in protein sequences?

I have a revised manuscript sent for publication and the reviewer had asked from me insert the percentage of homology of protein sequences then discuss, however, I discussed the DNA sequences...

04 April 2016 3,661 6 View

Does anyone have experience with LAMP protocol instead of PCR assay?

I mean what are the principles of LAMP and How can I prepare my Lab for it ?

04 April 2016 3,634 2 View

What are the advantages of using amino-acid sequences in comparison to using nucleotide sequences for phylogenetic analysis?

I need know how can I use and interpret the amino acid sequences for phylogeny for the same homologous region

03 March 2016 7,420 16 View

I need a teaching video for Glanders and Strangles in equine describing epidemiology, clinical signs, diagnosis and control?

I need this video to help me in making a lecture for 2nd year course

03 March 2015 5,967 3 View

Can I deposit the two different sequences of PPR virus regarding F and N genes in Genbank under the same accession number?

I have two sequences of PPR virus of two genes (F and N genes) (complete Cds).

10 October 2014 4,965 4 View

Does anyone have experience with the Kappa test?

I've data for diagnosis of mastitis by bacterial culture and PCR. I need make agreement inbetween by kappa test

09 September 2014 6,824 2 View

What is the ideal statistical analysis test to describe the association between results of PCR and bacterial culture in raw milk?

I have been examining milk from dairy cows and buffaloes for sub clinical mastitis. The obtained results were positive for PCR and bacterial culture. I want to know if there is association between...

01 January 2014 4,843 10 View

How I can deal, compare, and discuss the results of PCR and histology for detection of neethling virus in formalin fixed skin samples?

I examined the use of formalin fixed skin biopsy in detection of neethling virus by PCR and histology. The obtained results revealed that both assays detected the virus. So, how I can compare,...

10 October 2013 8,634 1 View