I mean what are the principles of LAMP and How can I prepare my Lab for it ?
Basically they are same.
The polymerase chain reaction (PCR) relies upon thermal cycling to achieve DNA strand separation, primer annealing and finally target extension. Loop-mediated isothermal DNA amplification (LAMP) uses a DNA polymerase enzyme with strand displacement activity to achieve strand deparation, primer annealing and target extension at a single (isothermal) temperature.
http://www.optigene.co.uk/support/
This link will answer anything about your queries.
Sir Elhaig, good morning,
This technology is used to enable the amplification of DNA quences with great specificity. The LAMP uiliza a DNA polymerase has atrand displacement activity of the antigen to be studied. It enables the isothermal amplification of DNA sequences and RNA order of 109 to 1010 at various times between 15 to 60 minutes.
They are made from four specific primers designed from the antigen to be studied drawn from six individual segments of the sequence to be amplified.
For the standards of laboratory functionality are needed to standardize the same with the legislation and attaching a thermal cycler, a cabin PCR, various pipettes, UV light and varied agents and dyes depend on the antigens to be studied.
I hope somehow have helped,
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