Is the amplification curve affected when I use RT-step on cDNA in real time one step RT-PCR?

While editing the cycling protocol for One Step real time RT-PCR, If used RT step (while sample used is cDNA). Will this affect the amplification as the temperature of RT step is 55 C (not...

03 April 2019 1,817 2 View

How can response to reviewer comment to remove a confused point or to prove a finding?

What the best way in Discussion section to remove a confusion on bacterial pathogen which has never recorded in the certain area and by PCR gave the same product size for a bacterium of our study...

04 May 2018 5,231 4 View

How can I manage large DNA sequences in DNA alignment?

During Blastin search, I found many sequences with Large sequence. (about 2105803 bp ). I faced a problem in adding them to DNA Alignment and in each time a message appeared "Warning: about to...

02 March 2018 2,076 7 View

Is there a site helps me to draw a map for use in epidemiological study to illustrate the geographical distribution of the disease?

I am working on epidemiological study, which aimed to study risk factors of infectious diseases distribution, spread and re-emerge in endemic areas. For this I am asking how can I draw a map...

02 March 2017 9,751 0 View

What are advantages studying the percentage of homology in protein sequences?

I have a revised manuscript sent for publication and the reviewer had asked from me insert the percentage of homology of protein sequences then discuss, however, I discussed the DNA sequences...

03 April 2016 3,575 6 View

Does anyone have experience with LAMP protocol instead of PCR assay?

I mean what are the principles of LAMP and How can I prepare my Lab for it ?

03 April 2016 3,546 2 View

What are the advantages of using amino-acid sequences in comparison to using nucleotide sequences for phylogenetic analysis?

I need know how can I use and interpret the amino acid sequences for phylogeny for the same homologous region

02 March 2016 7,205 16 View

I need a teaching video for Glanders and Strangles in equine describing epidemiology, clinical signs, diagnosis and control?

02 March 2015 5,882 3 View

Can I deposit the two different sequences of PPR virus regarding F and N genes in Genbank under the same accession number?

I have two sequences of PPR virus of two genes (F and N genes) (complete Cds).

09 October 2014 4,876 4 View

Does anyone have experience with the Kappa test?

08 September 2014 6,734 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View