Is the amplification curve affected when I use RT-step on cDNA in real time one step RT-PCR?

While editing the cycling protocol for One Step real time RT-PCR, If used RT step (while sample used is cDNA). Will this affect the amplification as the temperature of RT step is 55 C (not...

03 April 2019 1,817 2 View

What are principles of Real Time PCR?

i need to know: Principles Protocol The best kits available with high efficiency results How to read and interpret the results?

31 December 2018 2,234 0 View

How can response to reviewer comment to remove a confused point or to prove a finding?

What the best way in Discussion section to remove a confusion on bacterial pathogen which has never recorded in the certain area and by PCR gave the same product size for a bacterium of our study...

04 May 2018 5,231 4 View

How can I manage large DNA sequences in DNA alignment?

During Blastin search, I found many sequences with Large sequence. (about 2105803 bp ). I faced a problem in adding them to DNA Alignment and in each time a message appeared "Warning: about to...

02 March 2018 2,076 7 View

Is there a site helps me to draw a map for use in epidemiological study to illustrate the geographical distribution of the disease?

I am working on epidemiological study, which aimed to study risk factors of infectious diseases distribution, spread and re-emerge in endemic areas. For this I am asking how can I draw a map...

02 March 2017 9,751 0 View

What are advantages studying the percentage of homology in protein sequences?

I have a revised manuscript sent for publication and the reviewer had asked from me insert the percentage of homology of protein sequences then discuss, however, I discussed the DNA sequences...

03 April 2016 3,575 6 View

Does anyone have experience with LAMP protocol instead of PCR assay?

I mean what are the principles of LAMP and How can I prepare my Lab for it ?

03 April 2016 3,546 2 View

What are the advantages of using amino-acid sequences in comparison to using nucleotide sequences for phylogenetic analysis?

I need know how can I use and interpret the amino acid sequences for phylogeny for the same homologous region

02 March 2016 7,205 16 View

I need a teaching video for Glanders and Strangles in equine describing epidemiology, clinical signs, diagnosis and control?

02 March 2015 5,882 3 View

Can I deposit the two different sequences of PPR virus regarding F and N genes in Genbank under the same accession number?

I have two sequences of PPR virus of two genes (F and N genes) (complete Cds).

09 October 2014 4,876 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

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02 March 2021 3,060 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View