Is the amplification curve affected when I use RT-step on cDNA in real time one step RT-PCR?

While editing the cycling protocol for One Step real time RT-PCR, If used RT step (while sample used is cDNA). Will this affect the amplification as the temperature of RT step is 55 C (not...

03 April 2019 1,817 2 View

What are principles of Real Time PCR?

i need to know: Principles Protocol The best kits available with high efficiency results How to read and interpret the results?

31 December 2018 2,234 0 View

How can response to reviewer comment to remove a confused point or to prove a finding?

What the best way in Discussion section to remove a confusion on bacterial pathogen which has never recorded in the certain area and by PCR gave the same product size for a bacterium of our study...

04 May 2018 5,231 4 View

How can I manage large DNA sequences in DNA alignment?

During Blastin search, I found many sequences with Large sequence. (about 2105803 bp ). I faced a problem in adding them to DNA Alignment and in each time a message appeared "Warning: about to...

02 March 2018 2,076 7 View

Is there a site helps me to draw a map for use in epidemiological study to illustrate the geographical distribution of the disease?

I am working on epidemiological study, which aimed to study risk factors of infectious diseases distribution, spread and re-emerge in endemic areas. For this I am asking how can I draw a map...

02 March 2017 9,751 0 View

What are advantages studying the percentage of homology in protein sequences?

I have a revised manuscript sent for publication and the reviewer had asked from me insert the percentage of homology of protein sequences then discuss, however, I discussed the DNA sequences...

03 April 2016 3,575 6 View

Does anyone have experience with LAMP protocol instead of PCR assay?

I mean what are the principles of LAMP and How can I prepare my Lab for it ?

03 April 2016 3,546 2 View

What are the advantages of using amino-acid sequences in comparison to using nucleotide sequences for phylogenetic analysis?

I need know how can I use and interpret the amino acid sequences for phylogeny for the same homologous region

02 March 2016 7,205 16 View

Can I deposit the two different sequences of PPR virus regarding F and N genes in Genbank under the same accession number?

I have two sequences of PPR virus of two genes (F and N genes) (complete Cds).

09 October 2014 4,876 4 View

Does anyone have experience with the Kappa test?

08 September 2014 6,734 2 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Which are the most recent implications of ESL Academic Discourse in language teaching?

Language teachers at UTP (Language Department) are increasingly interested in the role of academic papers in the development of language (English) teaching practices. Research papers in Academic...

22 February 2021 1,388 4 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Can I follow Researchers from Teaching and testing areas?

I want Researchers from Educational Measurement and Evaluation, relating to teaching, learning , academic performance and test validation

18 February 2021 4,757 13 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

Can any scholar assist me with an r code for calculating average bias for each parameter of a continuous distribution via simulation?

I want to perform a simulation study to examine the consistency property of parameters of a new distribution through the quantile function. The simulation study will contain many replicates (say...

10 February 2021 180 3 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

What are the contents of the supplication of the oppressed that Imam Al-Hadi (peace be upon him) teaches us?

What are the contents of the supplication of the oppressed that Imam Al-Hadi (peace be upon him) teaches us?

08 February 2021 6,791 5 View