How to polish the samples which are very prone to oxidation for metallography?

I am trying to polish the rare earth based sample for SEM analysis. But the sample is getting oxidized immediately . Can anyone suggest a way to polish these kind of samples?

27 January 2021 4,699 2 View

How do we solve optimization problems involving a maxima or minima in the objective function ?

I am trying to solve a multi-objective optimization problem using the Pyomo library in Python. The objective function includes operational optimization of an electrolyzer for hydrogen production...

11 January 2021 8,018 3 View

Any researchers working on Mucuna pruriens with respect to tissue culture?

15 November 2020 8,675 1 View

How can I confirm that a gene is downregulated in cancer cells through PCR?

A particular gene (gene A) is reported to be downregulated in cancer cells as compared to the normal cells. How can you prove this with the help of PCR?

27 September 2020 5,292 4 View

Why my absorbance values are in negative for antioxidant assay by dpph method ?

plant extract stock is 10 mg/mL from which I pipetted 20, 40, 60, 80, 100, 200 uL and made up to 3 mL using methanol then i added 1 ml of 0.004% dpph solution (dissolved in methanol) incubated in...

02 September 2020 1,218 2 View

Is 5ug/ml puomycin works for Huh7 cell lines?

I am trying to make KO in Huh7 cell line but after transduction even at 5ug/ml concentration Huh7-Ctrl cell haven't died. Could someone please recommend me the working concentration of Puromycin...

17 August 2020 5,427 2 View

How can I find the relation of cancer stem cells and tumor microenvironment?

in Gastric cancer both cancer stem cells and tumor microenvironment prevents the action of therapeutics and drugs. a. How can I find a co-relation between them? b. How can i evaluate their role...

06 May 2020 6,428 7 View

What should be the best Performance Metrics for SVR Model ?

I am getting the following results for my SVR Model using Python's Sci-kit library : SVR RESULTS - C AND GAMMA PARAMETERS OPTIMIZED BY GRID SEARCH RMSE = 0.17050234208788798 MSE =...

08 February 2020 6,017 2 View

Salicylic acid is poorly soluble in water, I dissolved in 65% ethanol but it is forming precipitation on storage. What to do? Which solvent is best?

It dissolved in 60-65% ethanol but after few hours if storage at 4 degree Celsius it is forming precipitation

19 January 2020 5,092 9 View

In plasmid DNA isolation, potassium acetate re-anneals the plasmid DNA. How exactly does this happen? And why it doesn't happen for chromosomal DNA?

Alkaline lysis method

10 December 2019 2,472 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View