Bhaskar Vemu

4 Questions 15 Answers 0 Followers

Questions related from Bhaskar Vemu

How do I separate cells grown on collagen for western blot and also how do I know if cells are confluent if grown on a translucent membrane?

Working on Caco 2 cells. I want to know about the cells grown on collagen for differentiation. Also, can I perform a lucifer yellow assay for assessing the permeability followed by western blot in...

09 March 2018 9,005 4 View

How do you change the retention time of a compound that it does not ionise (lack Pka) in HPLC?

I have been trying to standardise the compound flubendiamide but the retention time does not change on altering pH and other parameters. Also can anyone suggest deproteinizing agents?

11 July 2014 3,294 1 View

Why do my Balb 3T3 cells die after I try to subculture them from the transport plate into a new flask?

I am planning to work on 3T3 cell line. I acquired them in healthy condition but started dying with structural changes when I tried to sub culture it. Serum was complement inactivated and all the...

08 June 2014 10,037 13 View

Can anyone suggest if there are any FACS kits to count the T (sub sets) and B lymphocytes, NK cells in rat blood and spleen cell suspension?

I am planning to carry out immunotoxicity studies in pesticides and as a part of the study i want to measure the subset population and their variations, live and dead count using FACS. Is there...

08 June 2014 6,670 2 View