For Insulin signaling you have two alternatives and all depend in which final event is going to be more important for you. C2C12 cells are good to study events related to metabolism and cell contraction, but for cell signaling per se L6 is better, because the signaling pathways in this cells are more similar to normal muscle cells, as well as the cellular markers of differentiation. The problem with this last ones is that they not contract.

So it all depends in which final event is going to be more important to you. But if you check the literature and use as key words Insulin Signaling and L6 or C2C12, the first hint gives you the double of information as the second. This is because regarding cell signaling the L6 line is a more validated model. Check the works of Proffesor Amira Klip and her work in Glut 4 translocation using the L6 cell lines.

For insulin signalling pathway, you can study on L6(rat skeletal muscle cells) or C2C12 cells (mouse myoblast cell line). Both cell lines are used as cell models.

Thank you everyone.

I am planning to use skeletal myoblast and C2C12

For insulin signaling the best model is L6 myotubes, the signaling pathways in this cells are similar to primary culture of muscle cells, as well as the cellular markers of differentiation. The response to contraction activity is absent. C2C12 is a good model too, particularly if you want to study the role of calcium in insulin signaling

Thank you very much Anton. just starting with work and will see to that it is differentiated into myotubes.

I have started with C2C12 and HSMM.