Revathy Carnagarin

10 Questions 10 Answers 0 Followers

Questions related from Revathy Carnagarin

What is the significance of serum starvation when studying molecular signalling pathways especially Insulin signalling?

Why do researchers serum starve the cells before studying the molecular event in a cell line. I am planning to look at the Insulin signalling events in skeletal muscle cell lines, should I serum...

28 February 2015 7,095 5 View

Please can anyone tell how much PEDF is secreted from human skeletal muscles roughly?

Do anyone have an idea about PEDF secretion from skeletal muscle.

12 February 2015 7,679 1 View

Are phosphorylated proteins of insulin signalling pathway very labile?

I have run western blots and have got clean bands for AKT, phospho Akts and others but I am not able to get clear bands on repeating the western from the same samples around 3 weeks later. My...

31 December 2014 5,052 5 View

How do you get a band in a western blot for Insulin receptor beta and phosphorylated insulin receptor beta?

I have done my western blotting using C2C12 cell lysate sample but I am not able to get the band for the above. I am using Santa Cruz antibodies for the insulin receptor beta subunit and its...

15 December 2014 8,310 4 View

How can I find the transfection efficiency when we use commercially available non-fluorescent siRNA for transfection?

I have used commercially control and target siRNA from SantaCruz to perform transfection in C2C12 and HSMM. I performed western blot and the target protein was not expressed while the controls...

25 November 2014 1,988 15 View

What is the housekeeping protein for C2C12?

Housekeeping protein for mice myoblast cell lines

19 November 2014 2,340 3 View

How to avoid non specific band in western blot ?

i am using a polyclonal rabbit primary for detecting a 50KDa protein but keep getting nonspecific band around 30KDa and few others. I block using 5% non fat milk for one hour sample preparation by...

29 October 2014 3,273 7 View

What is the incubation time required to use insulin as an inducer to study its signalling pathway in skeletal muscle (C2C12 and HSMM)?

Please can anyone suggest the incubation time required for Insulin for studying the downstream phosphorylation of its substrate proteins of mouse and human skeletal muscle (C2C12 and HSMM)?

21 October 2014 249 3 View

Is immunoprecipitation required for western blot analysis of both phosphorylated and nonphosphorylated insulin receptor beta and IRS ?

I am checking the effect of various proteins on the insulin signalling

29 September 2014 8,066 5 View

Can anyone suggest a skeletal muscle cell line for studying the effect of a newly identified protein on insulin signal transduction?

I am planning to examine the effect of a new protein on insulin dependent phosphorylation events up to GLUT4 translocation. I am designing my entire study on this and plan to use C2C12 but I...

16 July 2014 5,886 5 View