Dear Bhavya Revathy,
I think you can find something from below link!
http://microbeonline.com/urease-test-principle-procedure-interpretation-and-urease-positive-organsims/
Urease from jack beans (Canavalia ensiformis) was the first enzyme ever purified and crystallised, an achievement of James B. Sumner in 1926, at a time when most scientists believed that it was impossible to crystallise enzymes. This earned Sumner the 1946 Nobel Prize in Chemistry. Today, crystallisation of proteins helps scientists to discover their structure and determine how they work. This knowledge permits the design of substances that interfere with enzyme action, such as the anti-AIDS drugs which inhibit the action of HIV’s enzymes or recent developments towards a possible rabies treatment .Please find enclosed protocol for nitrate test. You can refer following references to watch out for protocols..
Ainsworth C (2008) Locking the cradle. Science in School 8: 25-28. www.scienceinschool.org/2008/issue8/rabies
McGee H (2004) On food and cooking. London, UK: Hodder & Stoughton. ISBN: 0340831499
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