Can we find Urbach energy from the Tauc's plot itself? That means, by extrapolating the urbach tail to x axis of Tauc's plot..
Which can be ideal RNA markers for cell fractionation, as in for nuclear fraction and cytoplasmic one? I need to work on lncRNA localization in the different cell fractions. Thanks in Advance!!!
28 February 2022 6,846 1 View
Hi, I am trying to use a cytoplasmic nuclear fractionation kit which is employed for protein analysis. However, I need to analyze the RNA as well using the kit and was looking for any RNase...
21 February 2022 5,760 1 View
willing to publish a paper
03 June 2019 5,607 2 View
Why the output voltage of inverter is zero eventhough the pulses are coming correctly from dSPACE controller.
24 April 2018 4,792 5 View
The protocols available are optimised at ph 6.8 to 7.
07 September 2017 8,939 2 View
I have done F- EMSA and used 200nm of my DNA and titrated it with different concentration of my repressor protein. I found the kd to be 1.6 micro molar and I used prism sofware to fit my data with...
24 September 2015 5,477 3 View
Hi fellow researchers, am an newbie in protein purification and hence this question. I am working on a novel protein whose size is 17kda.it does not have cysteine. It's PI is around 7.83. I did a...
20 June 2015 2,793 5 View
Why do researchers serum starve the cells before studying the molecular event in a cell line. I am planning to look at the Insulin signalling events in skeletal muscle cell lines, should I serum...
27 February 2015 7,115 5 View
Do anyone have an idea about PEDF secretion from skeletal muscle.
11 February 2015 7,695 1 View
I have run western blots and have got clean bands for AKT, phospho Akts and others but I am not able to get clear bands on repeating the western from the same samples around 3 weeks later. My...
30 December 2014 5,078 5 View
How to apply magnetic field parallel to b axis of any crystal
29 July 2024 4,083 2 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
How can I define Planetary motion in ABAQUS CAE, how define motion of three rollers around a central axis and passive rotation about their own axis?
16 July 2024 4,145 1 View
In terms of CFD, we often analyze the stability of the error using Von-Neumann analysis, especially for FDM based problems. Should we follow the same approach for a compressible fluid flow using FVM ?
13 July 2024 6,296 5 View
How can I change marker axis line type to dotted in 1D Results/S-Parameters in CST Studio Suite?
10 June 2024 9,828 0 View
Good morning, I am running some Steered molecular dynamics in Amber 22 and want to set some restraints in the z-axis so as to imply certain distances from my ligand to its substrate. My goal is...
27 May 2024 7,082 1 View
In corrosion studies, if the X axis represents Exchange Current Density and the Y axis represents Equilibrium Potential, is the attached picture correct? The exchange current density is higher...
22 May 2024 7,836 0 View
I have calculated Y2SiO5 with monoclinic system (Y1-Y2 two site, unique Si site and O1, O2, O3, O4 and O5 five site). Thus, I have used these parameters as a input file descriptions; Monoclinic...
15 May 2024 9,072 2 View
I have created an axis using Datum points with a specific vector in Abaqus, intending to rotate a cylinder around this axis. However, in the Assembly module under Rotate Instance, after selecting...
09 May 2024 9,298 1 View
I have been doing experiments with 2 electrode EIS on Hydrogels, having the gel sandwiched between 2 copper plates. My results vary between material and composition. but in a lot of the Nyquist...
03 May 2024 8,062 0 View