Any one interested in publishing papers in fluid dynamics?

willing to publish a paper

03 June 2019 5,402 2 View

How can we perform the urease and nitrate tests for an alkalibacterium that grows only above pH 8?

The protocols available are optimised at ph 6.8 to 7.

07 September 2017 8,796 2 View

EMSA kd interpretation?

I have done F- EMSA and used 200nm of my DNA and titrated it with different concentration of my repressor protein. I found the kd to be 1.6 micro molar and I used prism sofware to fit my data with...

24 September 2015 5,366 3 View

Are phosphorylated proteins of insulin signalling pathway very labile?

I have run western blots and have got clean bands for AKT, phospho Akts and others but I am not able to get clear bands on repeating the western from the same samples around 3 weeks later. My...

30 December 2014 4,990 5 View

How do you get a band in a western blot for Insulin receptor beta and phosphorylated insulin receptor beta?

I have done my western blotting using C2C12 cell lysate sample but I am not able to get the band for the above. I am using Santa Cruz antibodies for the insulin receptor beta subunit and its...

14 December 2014 8,237 4 View

How can I find the transfection efficiency when we use commercially available non-fluorescent siRNA for transfection?

I have used commercially control and target siRNA from SantaCruz to perform transfection in C2C12 and HSMM. I performed western blot and the target protein was not expressed while the controls...

24 November 2014 1,757 15 View

What is the housekeeping protein for C2C12?

Housekeeping protein for mice myoblast cell lines

18 November 2014 2,251 3 View

How to avoid non specific band in western blot ?

i am using a polyclonal rabbit primary for detecting a 50KDa protein but keep getting nonspecific band around 30KDa and few others. I block using 5% non fat milk for one hour sample preparation by...

28 October 2014 3,198 7 View

Is immunoprecipitation required for western blot analysis of both phosphorylated and nonphosphorylated insulin receptor beta and IRS ?

28 September 2014 8,007 5 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How do I get spectral power distribution (SPD) of my photoluminescence sample to calculate chromaticity coordinates?

Hi, I have photoluminescence samples which I already characterized and from that I already obtained the steady-state photoluminescence (SSPL) property. I want to draw CIE chromaticity diagram to...

24 January 2021 7,770 1 View

How to rotate an anisotropic stiffness matrix to an orthotropic matrix of cancellous bone?

Hi everyone, recently i have a question about how to calculate the orthotropic elastic matrix of cancellous bone cube. I really need the help!!!! I applied six stress states to the cancellous...

21 January 2021 9,143 2 View

How to rotate crystal structure to match with match another structure of same compound/polymorph?

Say, I have two crystal structures of a particular organic molecule, the crystal structures are basically identical, apart from a rotation and redefinition of the lattice vectors/angles, a simple...

07 January 2021 3,406 5 View

No title

04 January 2021 3,293 1 View

Why am i getting a negative torque in ansys fluent for a vertical axis wind turbine?

I am trying to simulate a vertical axis wind turbine of darrieus model and i am getting a negative torque. I have followed frame motion, relative to adjacent cell method, and solution method as...

28 December 2020 1,170 3 View

Why a magnetic field oriented along spin axis cannot reverse spins in the sublattice of an antiferromagnetic in T = 0 K?

A permeability of an antiferromagnetic along the spin axis is zero in T = 0 K as it is written in any handbook of magnetism. Why a magnetic field oriented along spin axis cannot reverse spins in...

27 December 2020 7,513 6 View

Log and normal scales equation interpretation ?

Hello all I'm interested in deducing a graph I have two values, one increases exponentially which is taken on X axis and other value taken on y axis increases normally (normal scale) taken. Can I...

17 December 2020 8,290 2 View

Can anyone help me with modelling of cyclic triaxial test in plaxis?

I am planning to do as axis symmetry case. But which model would be more appropriate for modelling strain controlled cyclic triaxial test on both silty sand as well as polymer treated silty sand?...

04 December 2020 8,522 4 View

How to give noncollinear magnetism terms in INCAR for Double helical magnetic ordering in VASP?

Usually, the magnetic moments of noncollinear tags in the INCAR file look like MAGMOM = 0.0 1.0 0.0 if the atom has 1 unit of the magnetic moment along the b axis. But if we consider the magnetic...

19 November 2020 8,061 3 View