12 February 2015 1 8K Report

Do anyone have an idea about PEDF secretion from skeletal muscle.

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Urbach energy calculation directly from Tauc's plot?

Can we find Urbach energy from the Tauc's plot itself? That means, by extrapolating the urbach tail to x axis of Tauc's plot..

26 July 2023 423 0 View

RNA markers for cell fractionation?

Which can be ideal RNA markers for cell fractionation, as in for nuclear fraction and cytoplasmic one? I need to work on lncRNA localization in the different cell fractions. Thanks in Advance!!!

28 February 2022 6,846 1 View

Is their any RNase inhibitor that can be used along with the fractionation kit buffers which need not need autoclaving or pre-heating?

Hi, I am trying to use a cytoplasmic nuclear fractionation kit which is employed for protein analysis. However, I need to analyze the RNA as well using the kit and was looking for any RNase...

21 February 2022 5,760 1 View

Any one interested in publishing papers in fluid dynamics?

willing to publish a paper

03 June 2019 5,607 2 View

Control of STATCOM using dSPACE?

Why the output voltage of inverter is zero eventhough the pulses are coming correctly from dSPACE controller.

24 April 2018 4,792 5 View

How can we perform the urease and nitrate tests for an alkalibacterium that grows only above pH 8?

The protocols available are optimised at ph 6.8 to 7.

07 September 2017 8,939 2 View

EMSA kd interpretation?

I have done F- EMSA and used 200nm of my DNA and titrated it with different concentration of my repressor protein. I found the kd to be 1.6 micro molar and I used prism sofware to fit my data with...

24 September 2015 5,477 3 View

Any suggestions on protein aggregates during buffer exchange in size exclusion?

Hi fellow researchers, am an newbie in protein purification and hence this question. I am working on a novel protein whose size is 17kda.it does not have cysteine. It's PI is around 7.83. I did a...

20 June 2015 2,793 5 View

What is the significance of serum starvation when studying molecular signalling pathways especially Insulin signalling?

Why do researchers serum starve the cells before studying the molecular event in a cell line. I am planning to look at the Insulin signalling events in skeletal muscle cell lines, should I serum...

27 February 2015 7,115 5 View

Are phosphorylated proteins of insulin signalling pathway very labile?

I have run western blots and have got clean bands for AKT, phospho Akts and others but I am not able to get clear bands on repeating the western from the same samples around 3 weeks later. My...

30 December 2014 5,078 5 View

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