RNA markers for cell fractionation?

01 March 2022 1 7K Report

Which can be ideal RNA markers for cell fractionation, as in for nuclear fraction and cytoplasmic one? I need to work on lncRNA localization in the different cell fractions.

Thanks in Advance!!!

Praveesh Valissery

Most transcripts that code for Nuclear-encoded-mitochondrial proteins (NEMPs) localize to the cytosol with very little in the nucleus. Xist1, MEG3 and MALAT1 transcripts can be used to validate nuclear fractions. (see https://www.nature.com/articles/s41598-021-83541-1.pdf for specifics).

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Revathy Nadhan's questions See All

Urbach energy calculation directly from Tauc's plot?

Can we find Urbach energy from the Tauc's plot itself? That means, by extrapolating the urbach tail to x axis of Tauc's plot..

26 July 2023 423 0 View

Is their any RNase inhibitor that can be used along with the fractionation kit buffers which need not need autoclaving or pre-heating?

Hi, I am trying to use a cytoplasmic nuclear fractionation kit which is employed for protein analysis. However, I need to analyze the RNA as well using the kit and was looking for any RNase...

21 February 2022 5,760 1 View

Any one interested in publishing papers in fluid dynamics?

willing to publish a paper

03 June 2019 5,607 2 View

Control of STATCOM using dSPACE?

Why the output voltage of inverter is zero eventhough the pulses are coming correctly from dSPACE controller.

24 April 2018 4,792 5 View

How can we perform the urease and nitrate tests for an alkalibacterium that grows only above pH 8?

The protocols available are optimised at ph 6.8 to 7.

07 September 2017 8,939 2 View

EMSA kd interpretation?

I have done F- EMSA and used 200nm of my DNA and titrated it with different concentration of my repressor protein. I found the kd to be 1.6 micro molar and I used prism sofware to fit my data with...

24 September 2015 5,477 3 View

Any suggestions on protein aggregates during buffer exchange in size exclusion?

Hi fellow researchers, am an newbie in protein purification and hence this question. I am working on a novel protein whose size is 17kda.it does not have cysteine. It's PI is around 7.83. I did a...

20 June 2015 2,793 5 View

What is the significance of serum starvation when studying molecular signalling pathways especially Insulin signalling?

Why do researchers serum starve the cells before studying the molecular event in a cell line. I am planning to look at the Insulin signalling events in skeletal muscle cell lines, should I serum...

27 February 2015 7,115 5 View

Please can anyone tell how much PEDF is secreted from human skeletal muscles roughly?

Do anyone have an idea about PEDF secretion from skeletal muscle.

11 February 2015 7,695 1 View

Are phosphorylated proteins of insulin signalling pathway very labile?

I have run western blots and have got clean bands for AKT, phospho Akts and others but I am not able to get clear bands on repeating the western from the same samples around 3 weeks later. My...

30 December 2014 5,078 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View