Can anyone suggest good companies where I can buy custom made lentiviral plasmids for my gene of interest - I would like a construct with conditional (on, off) expression with and without GFP tag. The conditional expression cannot be achieved with TET promoter (I use tetracyclin for differentiation of my neuronal cells ) .
Thank you! You mean "AVIVA Systems Biology"? I see that they are specialized in antibody services but i cannot find plasmids in their offer. I have just found "Cambridge Biosciences" - anyone know any other similar, maybe cheaper companies ?
Unfortunately my gene is not in the ThermoScientific collection:(
Maybe check hier: GeneCopoiea
http://www.genecopoeia.com/product/lentiviral/
Nice one Li, Cyagen's Vectorbuilder seems like an awesome tool for custom vectors!
Thank you all for the suggestions. What you think - what will be the best expression induction system alternative to Tetracyklin response element? I would like to induce the expression of my gene in the context of autophagy and apoptosis but I use tetracyclin to differentiate my cell line (Myc oncogen expression is controled by Tet) so the most popular Tet induction system is not appropriate in my case.
SBI has cumate driven lentivectors: http://www.systembio.com/lentiviral-technology/cumate-switch-vectors/overview
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Applied Biological Materials Inc.
8-13520 Crestwood Place
Richmond, BC, Canada
V6V 2G2
http://www.abmgood.com/
We used the pCDH-MCS-T2A-copGFP vector (BioCat). Lentiviral production by System Biosciences (www.systembio.com). The experiment ios described in Meinhardt et al, J. Neurosci 2013.
Hallo Herr Koenen, ich habe damals die adenoviralen Konstrukte von der Firma Sirion Biotech in Martinsried herstellen lassen. Diese Firma bietet auch Lentiviren an. Kann für Sie gerne auch den Kontakt herstellen. Sie können die Firma auch im Internet besuchen unter www.sirion-biotech.com.
You can always make your own. See http://www.broadinstitute.org/rnai/public/ for protocols. These are where Thermo/Sigma get there plasmids
Dear - one question more -What packaging cell line is the best - HEK293T - is it well enough or is it better to invest in some other?
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