Maria Thomas

6 Questions 34 Answers 0 Followers

Questions related from Maria Thomas

Genotyping tests for inborn resistance to SARS-CoV-2?

As far as I understand, without a working version of ACE2, SARS-CoV-2 is essentially locked out of a person's immune system. Are there already some tests available on genetic forms of ACE2 that...

18 October 2020 3,240 2 View

What is known about the mechanisms of CAR/NR1I3 activation via bilirubin in human hepatocytes?

Thank you for any input!

11 July 2016 326 2 View

Which backbone and which modification should I choose for splice-switching oligonucleotide?

I wanto to order splice-switching oligonucleotide which should target pre-mRNA and force/induce exon skipping to be used in human cells in cell culture experiments. Which backbone (DNA or RNA) and...

20 January 2015 5,515 9 View

Apoptin is a potent inducer of apoptosis specifically in cancer cells - why is it still not used in the clinics?

Since the first report in 1997 it is known that the chicken anemia virus protein, apoptin, induces apoptosis specifically in human transformed and malignant cells but not in normal cells. There...

17 March 2014 2,064 23 View

How to prove functional activity of the TF-binding site within an intron identified via ChIP-Seq?

We´ve got data strongly indicating that TF regulates the gene of interest via direct binding within an intron and not in the promoter region. How can I prove the functional activity of such a...

13 January 2014 7,613 28 View

Survival curves for stable clone generation

Does anybody know a resource for the concentrations of different antibiotics (like Hygromycin, Blasticidin, G418 etc) which should be used for the generation of stable clones in different cell...

13 February 2013 1,441 9 View