Maria Thomas

13 Questions 53 Answers 0 Followers

Questions related from Maria Thomas

Genotyping tests for inborn resistance to SARS-CoV-2?

As far as I understand, without a working version of ACE2, SARS-CoV-2 is essentially locked out of a person's immune system. Are there already some tests available on genetic forms of ACE2 that...

18 October 2020 3,258 2 View

What is known about the mechanisms of CAR/NR1I3 activation via bilirubin in human hepatocytes?

Thank you for any input!

11 July 2016 345 2 View

Could someone share his/her experience in using SOMAmers reagents in detecting the same protein using Aptamers or antibodies?

Could someone share his/her experience in using SOMAmers (Slow Off-rate Modified Aptamer) reagents in detecting the same protein using Aptamers or antibodies? Thank you!

10 November 2015 3,916 0 View

Which backbone and which modification should I choose for splice-switching oligonucleotide?

I wanto to order splice-switching oligonucleotide which should target pre-mRNA and force/induce exon skipping to be used in human cells in cell culture experiments. Which backbone (DNA or RNA) and...

20 January 2015 5,525 9 View

Is someone aware of clinical studies on combating obesity with bile acids?

Thank you.

19 December 2014 9,439 4 View

Apoptin is a potent inducer of apoptosis specifically in cancer cells - why is it still not used in the clinics?

Since the first report in 1997 it is known that the chicken anemia virus protein, apoptin, induces apoptosis specifically in human transformed and malignant cells but not in normal cells. There...

17 March 2014 2,078 23 View

How to prove functional activity of the TF-binding site within an intron identified via ChIP-Seq?

We´ve got data strongly indicating that TF regulates the gene of interest via direct binding within an intron and not in the promoter region. How can I prove the functional activity of such a...

13 January 2014 7,626 28 View

Can ROS influence alternative splicing?

And how?

23 July 2013 8,823 3 View

Nutraceuticals library for (high-throughput) screening?

Is anyone aware of a commercially available "nutraceuticals" - compound library for screening in the cell lines?

13 May 2013 2,913 4 View

ChIP-Seq analysis for only a number of several defined promoters?

We apply successfully a ChIP - Sybr-Green method in our group for analysis of TF binding enrichment within single promoters. Can one apply ChIP-Seq approach not for a genome-wide analysis but only...

30 April 2013 4,087 19 View

Physiological control of alternative splicing?

I would very appreciate to get some hints and relevant references on the examples of physiological control of kinetic coupling during alternative splicing.

25 April 2013 8,114 1 View

Survival curves for stable clone generation

Does anybody know a resource for the concentrations of different antibiotics (like Hygromycin, Blasticidin, G418 etc) which should be used for the generation of stable clones in different cell...

13 February 2013 1,451 9 View

PPARα: A sensitive sensor of nutritional status to link energy balance with drug metabolism?

We had an important discovery: PPARα directly regulates expression and activity of CYP3A4, the major drug metabolizing P450 enzyme in humans. (Klein et al, CPT, 2012; Thomas et al, Mol Pharmacol,...

14 January 2013 3,294 3 View