What is the optimum HEK 293T cell seeding cells/cm2 for large scale culture and lentiviral production?

After over a decade of seeding small(er) scale 293T fresh from ATCC I can say with confidence that Invitrogen (HeLa based) chart never really worked out for me to obtain 70-80% for 293T, the...

28 January 2015 8,417 14 View