Karthigeyan Dhanasekaran

2 Questions 13 Answers 0 Followers

Questions related from Karthigeyan Dhanasekaran

Ca some one suggest the best protocol to purify centrosomes without any nuclear envelope/ER contamination.?

I have been following the Tim Stearns protocol described in Methods in Cell Biol (Vol 129; page 153, 2015 issue). The fractions seem fine when probed for gamma-tubulin and histones, but I find...

12 July 2017 9,599 0 View

Is it good to pick a sgRNA target sequence 80-100bp downstream of the actual site of insertion?

Currently I am trying to create a CRISPR line for a c terminal Eos fusion. My PAM sequence with a score of 90 in the design tool is 80 bp downstream of the stop codon. Is it fine to design my...

15 May 2015 3,374 3 View