Could you suggest me any Protocol working with dream taq buffer and polymerase?

01 February 2016 3,039 6 View

Can you suggest me intracellular p-STAT3 staining for peripheral blood monocytes or granulocytes ?

31 December 2015 4,107 4 View

Does anyone use flow cytometry for nitric oxide (NO) detection ?

09 October 2015 8,685 9 View

What are the surface marker differences between young and old neutrophils obtained from mouse ?

How can we separate young and old mouse neutrophils? What are these cell's significant characteristic? What kind of techniques do you use to separate these cells?

02 March 2015 5,974 5 View

Is there anyone working with EL4 cell line?

I injected 500000 cells per animal subcutaneously but I couldn't palpate the Tumor. We are at 17th Day. What is your experience about this type of tumor? How many days should I wait for get tumor?

02 March 2015 8,741 3 View

Can anyone give me any suggestions for fixing this western problem?

Here is our western results. We try to understand these band appear like this. Do you have any opinion for solving this problem?

01 February 2015 376 4 View

Is there a specific intracellular staining protocol for phosphorylated protein detection?

01 February 2015 6,562 11 View

Detection of small peptide molecules derived from immune proteins?

I am working with small peptide molecules derived from immune proteins. My previous experiments were about their function. For this reason, I synthesized them using their amino asid sequence....

01 January 1970 5,577 3 View

Transfection of THP-1 cells?

01 January 1970 9,018 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View