I am working with small peptide molecules derived from immune proteins. My previous experiments were about their function. For this reason, I synthesized them using their amino asid sequence. Nowadays, I wonder about their precence at patient's serum/biological fluid or cell culture media. Firstly, I gonna investgate them with LC-MS/MS. Can u suggest me some sensitive methods?
The most sensitive methods in this context seem to be immunochemical methods, such as ELISA or RIA. However, in this case, you need good (!) antibodies against your peptides first. This might be a difficult task, if validated, commercial antibodies are not available.
targeted LC-MS/MS experiments (towards your peptide) in albumin-depleted serum could be another option, in addition to immunochemical techniques as just pointed out by Michael. Your synthetic peptide could be coupled to a carrier such as ovalbumin or KLH and injected in a rabbit, hoping to get good antibodies to your peptide
LC MS/MS is the best option for you. As mentioned before by Bruno Lamonte, you can make antibody specific for this protein. However the most sensitive method for you is LC MS MS .
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biomolecules
- Peptides