Can anyone recommend a protocol for the synthesis of stable cell line?

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Can anyone explain why do we get diffused bands in western blot? and any trouble shooting techniques?

Hello, Everyone. I have some questions related to western blot as I am new to western blot. I tried to detect the LC3B, however I got diffused bands as I have attached in the file (Please find the...

10 November 2019 5,975 13 View

How much should we add primary antibody into 20 mL of 1% Skim milk for Western Blot?

Hello, Everyone! I currently start doing western blot experiment, but I am not really sure how much should I add primary antibody into 20mL of 1% Skim milk. Here is what I am doing: * I make 1%...

10 November 2019 5,506 9 View

Any recommended handling method for neuroblastoma SH-SY5Y cells?

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07 August 2019 8,524 9 View

Any good handling methods for SH-SY5Y cells?

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06 July 2019 1,489 1 View

Could anyone recommend me the standard protocol of the design of (microRNA or gene) primers for RT-PCR as a reference?

I am looking for a standard protocol of how to design a good primer for RT-PCR. Could anyone recommend me the standard protocol of the design of (microRNA or gene) primers for RT-PCR as a reference?

05 June 2019 6,880 6 View

Why the expression pattern in Real-time PCR keeps changing for the same primer?

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Which RNA extraction method provides better result between the extraction with QIAzol and that with TRIzol?

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10 November 2018 9,262 5 View

Possible Target genes or Primers for miRNA?

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08 September 2018 4,600 7 View

What somehow makes WST-1 cell proliferation experiment not working ?

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08 September 2018 7,793 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

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07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

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03 August 2024 2,730 1 View

T. H. Al-Noor

ttps://www.atcc.org/.../0C40E7796AEC4DAB9A657C41BA917C3D...

Joseph Capani Jr.

I assume by stable cell line you mean immortalized cell line. If this is the case, our lab has had good experiences with HEK and 3t3 cell lines - both of which have a large body of literature describing various handling protocols.

Sokviseth Moeng

T. H. Al-Noor Thanks so much for your contribution. I will check it out.

Joseph Capani Jr. I would appreciate if you share some useful protocols with me.

Sokviseth Moeng http://www.bowdish.ca/lab/wp-content/uploads/2014/11/Culture-of-HEK-cells.pdf for example. Many more can be found via Google Scholar