Sokviseth Moeng

10 Questions 28 Answers 0 Followers

Questions related from Sokviseth Moeng

Can anyone explain why do we get diffused bands in western blot? and any trouble shooting techniques?

Hello, Everyone. I have some questions related to western blot as I am new to western blot. I tried to detect the LC3B, however I got diffused bands as I have attached in the file (Please find the...

11 November 2019 5,898 13 View

How much should we add primary antibody into 20 mL of 1% Skim milk for Western Blot?

Hello, Everyone! I currently start doing western blot experiment, but I am not really sure how much should I add primary antibody into 20mL of 1% Skim milk. Here is what I am doing: * I make 1%...

11 November 2019 5,350 9 View

Any recommended handling method for neuroblastoma SH-SY5Y cells?

Good afternoon, I have some question related to the handling method for neuroblastoma cells SH-SY5Y. First, I grew the cells with EMEM medium (10% FBS and 1% Penicillin/Streptomycin). The cells...

08 August 2019 8,447 9 View

Can anyone recommend a protocol for the synthesis of stable cell line?

I am currently looking for the protocol of making the stable cell line. Can anyone recommend one for me? Really appreciate your help!!!

07 July 2019 7,708 5 View

Any good handling methods for SH-SY5Y cells?

Hello, Everyone! I have some questions related to the SH-SY5Y cell handling. I grow my SH-SY5Y cells in cell culture dish with EMEM (FBS+, P/S+)cell culture media. One day after cell plating,...

07 July 2019 1,407 1 View

Why the expression pattern in Real-time PCR keeps changing for the same primer?

Good morning, Everyone! I have some questions related to Real-time PCR. I have checked the expression of a microRNA (microRNA primer) in monolayer and spheroid cells. The first time I run the...

06 June 2019 4,382 13 View

Could anyone recommend me the standard protocol of the design of (microRNA or gene) primers for RT-PCR as a reference?

I am looking for a standard protocol of how to design a good primer for RT-PCR. Could anyone recommend me the standard protocol of the design of (microRNA or gene) primers for RT-PCR as a reference?

06 June 2019 6,808 6 View

Which RNA extraction method provides better result between the extraction with QIAzol and that with TRIzol?

Firstly, I have extracted RNA samples by using QIAzol, and then I also tried to use TRIzol to extract RNA samples. Can anyone tell me which RNA extraction method is better between the extraction...

11 November 2018 9,190 5 View

Possible Target genes or Primers for miRNA?

Hi everyone, Could you tell me how to find possible target genes or primers for a specific microRNA when we perform RT-PCR? My experiment is I transfected cells with a specific miRNA, then I...

09 September 2018 4,533 7 View

What somehow makes WST-1 cell proliferation experiment not working ?

Hi Everybody, I have some questions related to WST-1 cell proliferation assay. I did a WST-1 assay experiment with my pancreas cancer cell line in 96-well plate. First, I plated the cells in...

09 September 2018 7,717 0 View