How to extract protein after trizol RNA and back extraction of genomic DNA?

I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA...

13 January 2021 1,566 2 View

Where can I find solar irradiation data with high resolution?

I am trying to generate a 1 day solar irradiation profile. Any suggested high resolution(possibly in minutes) source for the same?

06 December 2020 5,976 18 View

How to implement only a Gate with activation sigmoid or tanh in keras?

I want to implement only a Gate similar to forget gate or output gate of LSTM cell. Mind it...!!! I am not implementing the entire LSTM cell rather I have certain input and previous output and I...

22 September 2020 8,236 3 View

Could someone help me interpret the following PMF curve, especially the physical reason behind the multiple potential wells?

I am simulating the pulling of two nanoparticles in a polymer. I get the attached PMF curve, showing a secondary (and much deeper) potential well. I was wondering if there is a physical...

19 September 2020 7,504 5 View

How to determine relationship among the independent variables to get lowest MSE (Mean Squared Error) in prediction?

I have a dependent variable A, which can be perceived from B, C and D. So to say A = function(B,C,D). B, C, D results in A that is known to us. However, I don't know whether B,C and D follow a...

28 June 2020 6,332 6 View

IS it Necessary for Primers in Taq-Man PCR to be HPLC Purified?

I want to set up a taq man reaction. I want to know that in taq man reaction I have to order HPLC purified Primers or ordinary primers are adequate for me. If you have experience in taq man,...

23 June 2020 5,511 3 View

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We're looking at storing serum and plasma samples from a clinical trial. I've looked at various papers, but the only place where I found some hazy answer was in the supplementary. But I'm hoping,...

04 March 2020 8,858 3 View

How to calculate the required motor HP to run a machine?

Hello every one, Can some body help me in calculating the required HP for motor to run the machine which includes transmission system with 2 Nos of shaft and cutting blades.

28 December 2019 9,788 3 View

Is there any benchmark dataset for distributed camera networks?

I am particularly looking for dataset containing cameras capturing videos in a distributed setup. The videos, captured by multiple cameras have to be correlated. Is there any benchmark dataset on...

22 November 2019 9,567 3 View

Could anyone recommend a protocol to estimate the concentration of residual antibiotics in a tissue allograft?

I have been trying to detect and quantify residual antibiotics left over from processing of my tissue allografts. Could anyone help me with the same?

01 August 2019 8,465 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View