Can anyone recommend a good assay kit for PDH activity?

22 October 2015 1 6K Report

Im looking for an assay to measure the PDH activity in cancer cell lines. Has anyone used a PDH activity assay which works well.

Thanks in advance!

Chithan C Kandaswami

Dear Devina,

PDH activity can be determined employing standard spectrophotometric assays.

http://www.jbc.org/content/241/20/4694.full.pdf+html I. ISOLATION, PURIFICATION, AND PROPERTIES OF PYRUVATE DEHYDROGENASE COMPLEX OF PIG HEART MUSCLE

http://www.jbc.org/content/256/13/6583.full.pdf

http://www.ncbi.nlm.nih.gov/pubmed/6839008

http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/1/mak183bul.pdf ...2014 Sigma assay kit

https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/General_Information/pyruvate_dehydrogenase.pdf

http://www.ufrgs.br/biofisica/Bio10003/PDHassay.pdf

Colorimetric assay kit:

http://www.biovision.com/pyruvate-dehydrogenase-pdh-activity-colorimetric-assay-kit-7809.html

PDH assay in cancer cells:

http://www.cancerandmetabolism.com/content/3/1/7

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