I want to extract RNA from chicken gizzard tissue. But currently I don't have a homogenizer. What parameters should I use, like the power, Ton, Toff, total time etc, to effectively homogenize the crushed tissue without hampering RNA quality?
I have a doubt on in-silico modeling. I want to cyclize a linear peptide sequence by in silico. Which is more favorable method I don't understand. There is 2 pathways- 1. I can cyclize a linear...
12 June 2024 2,449 2 View
I have obtained 100 ul of pre-amplified cDNA using the SSIV SINGLE CELL PRE-AMP KIT. Now i want to make a dilution because my samples are limited and i cannot directly use the cDNA. So what...
22 May 2024 6,017 3 View
I need to calculate dG MM/GBSA energy through the MD simulation trajectories. From RMSD values I selected last 400 frames from MD trajectories. I ran thermal_mmgbsa.py script, but it calculates dG...
22 May 2024 10,076 5 View
pI of my protein is 4.1. I am using Ni coumn to purify it . Which binding buffers will be good? Even the pI is low can it still bind to nucleic acid? How do I know?
04 May 2024 8,535 2 View
I am keeping my cell line for drug treatment and monitoring its effect at 24 hrs, 48 hrs and 72 hrs? Should I change the medium every 24 hrs and give fresh drug treatment or just leave the medium...
01 May 2024 10,011 3 View
Protein:DNA charge ratio plays an important role in forming condensates. In most cases the N/P ratio ~1 is favored. However how this ratio depends on the concentration of the protein is not...
20 April 2024 4,753 0 View
The "blue economy" refers to the sustainable use of ocean resources for economic growth, improved livelihoods, and jobs while preserving the health of ocean ecosystems. It encompasses a wide range...
19 April 2024 7,456 2 View
I've prepared the following ceramics: Sample ID = Composition LMT = Li2Mg3TiO6 LMCT = Li2(Mg2.88Ca0.12)TiO6 LMCT-MN = Li2(Mg2.88Ca0.12)(Ti0.95(Mg1/3Nb2/3)0.05)O6 LMT-MN =...
07 April 2024 4,717 2 View
AI has immense potential to address various aspects of climate change by enhancing our understanding of the climate system, optimizing resource usage, and developing innovative solutions. Here are...
26 March 2024 958 1 View
Have anyone tried to measure calprotectin from feces sample of mice using ELISA
03 February 2024 6,049 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View