I have a little time for optimization and therefore will be happy if someone could provide an experimentally validated sequence of primers for RT-PCR amplification of human housekeeping gene RPL13A with the use of QUIAGEN RT2 SYBR green master mix. The cycling is two stage with 15sec denaturation and 1min annealing in 60C. The company wants around 220EUR for a 200 reaction tube so I believe it is a daylight robbery and would like to order synthesized oligos that will be compatible with that kit. I hope they do not introduce nothing more to the assay and it is just oligonucleotide in a buffer.
Biorad sell a predesigned SYBR as well as probe assay for this marker (PrimePCR). They have been designed by Jo Vandesompele and Jan Hellemans from Ghent University, so will definitely work well. Assays are quite inexpensive at around £70 for a SYBR Green assay that will last a long time. I have evaluated a number of their assays and they are all very efficient, at near 100%.
It's true, other companies will sell you validated primers for a lower cost. Try here: http://www.genecopoeia.com/product/search/result.php?tax_id=9606&key=rpl13a&x=0&y=0&prt=19&field=0&cid=
Alternatively, you can design your own fairly easily. Here's a detailed protocol I wrote: https://sites.google.com/site/pasteuralternativecareers/research-protocols/design-your-own-qpcr-primers
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