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Questions related from Mark Livingstone
Traditional qPCR can give results in about an hour to detect/quantify DNA or RNA from various sources (bacteria, viruses, human, etc). I'm interested to know how you would use a machine that could...
25 March 2020 6,013 6 View
TriLink is making custom Cy5-labelled mRNAs encoding GFP, so I think it might be interesting to use a 2-color flow cytometry approach with various 5'UTRs and 3'UTRs to look at mRNA translation...
03 June 2015 2,805 3 View
Has anyone compared various transfection reagents comparing transfection efficiency across multiple cell lines...specifically for modified mRNAs? Also, I'm interested to know if anyone...
15 May 2015 5,197 7 View
Someone in our lab would like to continue using DNAStrider but has switched to a Mac laptop running OS10.8. Is there a similar program that is OSX compatible?
20 May 2014 5,377 14 View
Doing 5'-3' RACE and oligocapping combined with sequencing, I can see the primary transcriptional start site for an mRNA change from position 2 to position 30 to position 205 on the same mRNA...
19 February 2014 3,551 2 View
In an effort to reduce costs I ordered custom primers for PCR amplification prior to running on the Hiseq2000 and there were no clusters. I trusted our platform's technician blindly regarding what...
12 November 2013 3,658 10 View