Mark Livingstone

6 Questions 152 Answers 0 Followers

Questions related from Mark Livingstone

What applications can you think of for ultra fast qPCR ?

Traditional qPCR can give results in about an hour to detect/quantify DNA or RNA from various sources (bacteria, viruses, human, etc). I'm interested to know how you would use a machine that could...

25 March 2020 6,020 6 View

Has anyone heard of studying mRNA translation control by flow cytometry?

TriLink is making custom Cy5-labelled mRNAs encoding GFP, so I think it might be interesting to use a 2-color flow cytometry approach with various 5'UTRs and 3'UTRs to look at mRNA translation...

03 June 2015 2,814 3 View

What is the best transfection reagent for chemically-modified mRNAs (pseudouridine, 5-methylcytidine, ARCA)?

Has anyone compared various transfection reagents comparing transfection efficiency across multiple cell lines...specifically for modified mRNAs? Also, I'm interested to know if anyone...

15 May 2015 5,205 7 View

Can anyone suggest a DNAStrider alternative for OSX 10.8?

Someone in our lab would like to continue using DNAStrider but has switched to a Mac laptop running OS10.8. Is there a similar program that is OSX compatible?

20 May 2014 5,383 14 View

Any advice for qPCR validation of relative expression of transcriptional start site variants?

Doing 5'-3' RACE and oligocapping combined with sequencing, I can see the primary transcriptional start site for an mRNA change from position 2 to position 30 to position 205 on the same mRNA...

19 February 2014 3,556 2 View

Is there a problem with my homemade HiSeq primers?

In an effort to reduce costs I ordered custom primers for PCR amplification prior to running on the Hiseq2000 and there were no clusters. I trusted our platform's technician blindly regarding what...

12 November 2013 3,677 10 View