How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

What online teaching new topics to address as a phd topic?

Greetings, I have just started my phd in the field of english language studies, Im currently interested in online teaching, e-learning but mostly english language pedagogy. what are the new...

16 November 2020 4,045 9 View

Hi There any one can help on the case study below?

04 October 2020 7,234 1 View

After COVID 19, Can health be a decisive point for urban regeneration?

Will there be a return to suburbia after social distancing in city centres? From NY to Bombay how will people react to density and habitations after the tiny assassins take over us?

05 May 2020 6,256 6 View

Best strategy to transfect large genes in mammalian cells?

I have an insert ( ORF 6,9kb long) that I want to transfect in a human colon cancer cell line. I initially wanted to do with lentivirus, since I could have a stable expression of my gene and...

22 April 2020 3,277 3 View

What to do with coincident features for Geographically weighted regression?

I am sorry if this is a simple question. I am trying to conduct a GWR for hedonic analysis of hotel room rates. For OLS I have conducted analysis including different room types (e.g. double,...

16 April 2020 7,062 3 View

Can a conjugated isotype antibody be used in cell culture function blocking antibody experiment?

I have a PE conjugated mouse k isotype antibody..I would like to use it as control and add to cell culture .I would like to know whether conjugate will interfere in cell culture...

22 January 2020 607 2 View

What should be a loading control in condition media for zymography?

I want to do a zymography in condition media from cancer cell line...I would like to know what should be a loading control for condition media specific for zymography? Whether loading control can...

13 November 2019 1,697 1 View

Why c18 column isn't working?

I tried to work using same method I have worked before. But now not getting any peak. I am confused. Unable to understand what's happening. What can I do now?

02 October 2019 9,790 6 View

What is the best way to analyze interview question with scale answer from 1-5?? could I use likert even my research is qualitative?

I have a structured interview to test a hypothesis, one of the questions required an answer from 1 to 5 .. I don't know how to analyze this? any recommendations? @qulitative analysis @Structured...

21 February 2019 6,307 11 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View