Ishmat Ara Yasmin

8 Questions 9 Answers 0 Followers

Questions related from Ishmat Ara Yasmin

What is the effective concentration of pipercillin to be used in cell culture?

Can anyone please help me in deciding the concentration to be used of piperacillin sodium and tazobactum sodium in cancer cell line to prevent contamination?

15 January 2021 1,216 1 View

How to add a scale bar in ImageJ ?

I have taken images in 10X and 20X in Nikon Eclipse TE 2000S microscope. I want to know how to add scale bar? Can anyone please help me how to add scale bar using pixel?

12 April 2020 5,223 6 View

Can a conjugated isotype antibody be used in cell culture function blocking antibody experiment?

I have a PE conjugated mouse k isotype antibody..I would like to use it as control and add to cell culture .I would like to know whether conjugate will interfere in cell culture...

23 January 2020 779 2 View

What should be a loading control in condition media for zymography?

I want to do a zymography in condition media from cancer cell line...I would like to know what should be a loading control for condition media specific for zymography? Whether loading control can...

14 November 2019 1,847 1 View

How to add scale bar?

I have done one sphere forming assay .I have taken images using mobile of the microscopic view!kindly let me know how to add scale bar to those images if the photo is taken by mobile...

14 September 2019 9,550 0 View

Why cloning is not working with different condition

I am trying to clone 3 genes of size 1.041kb ,438 bp,387 bp into a plasmid of 4.7kb size.I have my genes cloned in another expression vector.I did restriction digestion for 30min at 37 C with NEB...

01 January 1970 9,248 5 View

What does a negative value means in protein estimation by nanodrop 1000?

I have measured protein concentration using nanodrop 1000 at 280nm where I took 1ul of samples and took lysis buffer as blank. The software shows 1 abs =1 mg/ml . And I have got nanodrop values...

01 January 1970 7,862 5 View

Can anyone please suggest about ChIP qpcr calculation by percent input method

i sonicated my samples (Control,treatment). From there i had taken 100 ul samples and diluted with 400 ul of IP dilution buffer. from there i have taken 50ul as input and stored. from the...

01 January 1970 2,034 0 View