Who uses Tetra-Arms PCR method ? Who can help us in using the method specific in using primers(outer & Inner Primers) ??
Amplification refractory mutation system ARMS-PCR is being used to detect two alleles simultaneously by using a single PCR reaction system, which does not require restriction endonuclease and can distinguish whether the alleles are pure or not.
ARMS-PCR is a method we use to detect mutations/study polymorphisms . during amplification process we will get different size bands and depending on the bands we can interpret the result as homzygous / heterozygous .
it depends on what you are exactly working on , you can find online tools but don't trust them 100% .
Good luck
Dear ياسمين
you can use this tool but finally you should optimise the primer practically, because primer designs by bioinformatic tool for ARMS_PCR some time fail in real discrimination of the SNPs in the lab.
https://snp.biotech.edu.lk/arms.php
Arms-PCR method is used for detection of polymorphic alleles like insertion Deletions polymorphism. You can design your primers using primer3 or any other bioinformatics tool and then optimize them. Or you can refer to companies like Qiagen who have already designed and optimized such primers. They have a validated kit for InDels analysis.
Good Luck
Researchers and labs in the disciplines of genetics, genomics, and molecular biology typically employ the Tetra-Arms PCR technique for SNPs (Single Nucleotide Polymorphisms) identification. SNPs and other genetic variants, such as them, may be specifically amplified and identified in DNA samples using this technique. It is employed in many different contexts, including as population genetics studies, forensic analysis, paternity testing, and disease research.
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