For the identification of jellyfish, mCOI, ITS1-2, 16S, 18S, and 28S primers have been used in different published research papers. Among them, I am considering selecting the primers mCOI and ITS1-2. There is an extensive database of COI primers in GenBank, which could be helpful in the future. Also, I have universal ITS1-2 primers available to do this sequencing. However, any other comment or suggestion would be valuable insight for selecting the primers. Thank you in advance.
I would do both. One is mitochondrial, the other nuclear, which is nice. Both work well on Cone Snails and Mammals. CO1 is great for most anything.
Thank you so much, Patrice Showers Corneli. I think CO1 will also help with comparative phylogenetic analyses. We would be able to distinguish phylogenetic differences among this species' variants.
For DNA barcoding of jellyfish species identification, selecting mCOI and ITS1-2 primers is an excellent choice. The mCOI (cytochrome c oxidase I) gene is widely used for species identification due to its high resolution and extensive reference database in GenBank, facilitating accurate species comparison and future research utility. The ITS1-2 regions (Internal Transcribed Spacer) offer high variability, making them effective for distinguishing closely related species, and you already have universal ITS primers available for sequencing. Together, these primers provide a robust approach for identifying jellyfish species, leveraging both mitochondrial and nuclear markers to enhance accuracy and reliability.
Devrim Can Ates Thank you so much for your informative answer.
Mst Shamim Ara Supty For DNA barcoding of jellyfish for species identification, the primer pair LCO1490 and HCO2198 is highly recommended. These primers target the mitochondrial cytochrome c oxidase subunit I (COI) gene, which is a standard marker used in molecular taxonomy due to its high interspecies variability and low intraspecies variability. The forward primer LCO1490 and the reverse primer HCO2198 have been extensively validated and are known for their reliability and reproducibility in amplifying the COI region across a wide range of metazoans, including jellyfish. The amplified COI sequences can then be compared against reference databases such as the Barcode of Life Data Systems (BOLD) for accurate species identification, contributing significantly to the study of jellyfish biodiversity and taxonomy.
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