I need enzymes that can work with my in-house PCR buffer. Please suggest if there is any good DNA polymerase and reverse transcriptase, I also do not have a lot of funds so I would be really grateful if price is mentioned along with.
I worked with RevertAid RT Reverse Transcription Kit from thermofischer and it works really well. It a bit expensive (652 euros without transportation).But you can perform 500 reactions with it.
For the DNA polymerase it depends on how big is the size of the sequence you want to amplify.