Nadia Jamil

17 Questions 37 Answers 0 Followers

Questions related from Nadia Jamil

What is the proper method for cleaning of combs for agarose gel electrophoresis?

Can we wash it by boiling it in water if the visible stains of ethidium bromide are not washed after rinsing with water and then 70% ethanol?

08 August 2018 8,911 3 View

Which software is better for Statistical Analysis, R or SPSS?

I need to analyze my data statistically, and I need a user friendly software with international recognition for publication, please suggest any software for that purpose. It would be great help if...

07 July 2018 3,864 12 View

What does number written between sequence of nucleotide means?

If there is a number superscript on the sequence of a primer what does it means? e.g. T153AGCT etc

12 December 2017 10,053 1 View

Can anyone recommend me any tools or software's for Viral In silico PCR and predicting DNA bands?

I have seen following...

12 December 2017 1,240 4 View

Does anyone know what is Global Impact Factor (GIF)?

should we publish in Journals having GIF, as they are charging very few amount of fee.

11 November 2017 7,923 0 View

Does anyone know about Academic Research International Journal?

Is this journal recognized or not? it is showing that it is indexed in Index Copernicus.

11 November 2017 4,770 6 View

What is the best method for Enhancing Gene Expression?

What is best method for enhancing the expression of a gene if you need a protein, is it increasing copy no or removing control from it or anything else?

11 November 2017 7,331 5 View

My PCR band disappeared after optimization, what should I do?

I ran a PCR reaction and got my desired band at 800 bp with other non specific bands (reaction vol 25 ul) after optimization I got 800 bp band clear (reaction vol 25 ul). Then I went for bulk...

10 October 2017 6,510 8 View

How quickly the PCR primer deteriorates?

I used to use PCR primers even after 3-4 months and they worked, but now something seems to be wrong with them, they stop working after few runs. I am totally clueless about it.

10 October 2017 1,474 6 View

What is the difference between normal primers and primers for cloning?

I need to work on expression of a gene. can anyone tell me about the difference of these primers?

10 October 2017 6,562 4 View

Can we use any reverse transcriptase enzyme with any type of buffer?

when we use reverse transcriptase do we have to use specific buffer with that or any taq buffer can also work with that?

10 October 2017 1,715 3 View

Can anyone please suggest a good DNA polymerase and reverse transcriptase?

I need enzymes that can work with my in-house PCR buffer. Please suggest if there is any good DNA polymerase and reverse transcriptase, I also do not have a lot of funds so I would be really...

10 October 2017 7,757 4 View

Result of my BLAST says no significant similarity found, what does it mean?

Hi there I isolated bacteria from serum sample did the extraction of DNA for bacteria, followed by PCR. PCR band was of 100bp I sent it for sequencing. The results were approx 43 bp forward primer...

08 August 2017 6,396 10 View

What is the difference in Bst DNA Polyemrase and Bsm DNA Polymerase?

which one is better in performing Loop mediated isothermal amplification (LAMP)

01 January 1970 6,730 0 View

Can some one from Karachi University tell me how to put your name in PCD List for HEC requirement?

HEC Scholars from Pakistan

01 January 1970 4,750 3 View

How to choose an ATCC strain as control?

If there are more than one number available. How can we find what is the difference between these e.g. Bacillus licheniformis has so many numbers.

01 January 1970 6,454 3 View

How we can standardize molar solution of EDTA?

can anyone tell me that how do we confirm that molarity of any solution of ours is exactly what we have made after calculations? e.g. how can we confirm that a 5M EDTA solution is actually 5M or...

01 January 1970 4,828 3 View