when we use reverse transcriptase do we have to use specific buffer with that or any taq buffer can also work with that?
You'd better use the corresponding buffer in case of unknown circumstances.
In case of commercial assays, I would recommend to use appropriate buffer to ensure optimal performances. An other RT buffer could also be used I think but performances might be lower ...
Thank you Maxime Pichon and Deyuan Dong.
Can we wash it by boiling it in water if the visible stains of ethidium bromide are not washed after rinsing with water and then 70% ethanol?
07 August 2018 8,943 3 View
I need to analyze my data statistically, and I need a user friendly software with international recognition for publication, please suggest any software for that purpose. It would be great help if...
06 July 2018 3,888 12 View
If there is a number superscript on the sequence of a primer what does it means? e.g. T153AGCT etc
11 December 2017 10,081 1 View
I have seen following...
11 December 2017 1,280 4 View
should we publish in Journals having GIF, as they are charging very few amount of fee.
10 November 2017 7,949 0 View
Is this journal recognized or not? it is showing that it is indexed in Index Copernicus.
10 November 2017 4,813 6 View
What is best method for enhancing the expression of a gene if you need a protein, is it increasing copy no or removing control from it or anything else?
10 November 2017 7,366 5 View
I used to use PCR primers even after 3-4 months and they worked, but now something seems to be wrong with them, they stop working after few runs. I am totally clueless about it.
09 October 2017 1,507 6 View
I need to work on expression of a gene. can anyone tell me about the difference of these primers?
09 October 2017 6,608 4 View
I ran a PCR reaction and got my desired band at 800 bp with other non specific bands (reaction vol 25 ul) after optimization I got 800 bp band clear (reaction vol 25 ul). Then I went for bulk...
09 October 2017 6,542 8 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
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01 August 2024 5,460 5 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...
20 July 2024 1,440 3 View