Can we use any reverse transcriptase enzyme with any type of buffer?

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What is the proper method for cleaning of combs for agarose gel electrophoresis?

Can we wash it by boiling it in water if the visible stains of ethidium bromide are not washed after rinsing with water and then 70% ethanol?

07 August 2018 8,843 3 View

Which software is better for Statistical Analysis, R or SPSS?

I need to analyze my data statistically, and I need a user friendly software with international recognition for publication, please suggest any software for that purpose. It would be great help if...

06 July 2018 3,787 12 View

What does number written between sequence of nucleotide means?

If there is a number superscript on the sequence of a primer what does it means? e.g. T153AGCT etc

11 December 2017 9,986 1 View

Can anyone recommend me any tools or software's for Viral In silico PCR and predicting DNA bands?

I have seen following...

11 December 2017 1,164 4 View

Does anyone know what is Global Impact Factor (GIF)?

should we publish in Journals having GIF, as they are charging very few amount of fee.

10 November 2017 7,851 0 View

Does anyone know about Academic Research International Journal?

Is this journal recognized or not? it is showing that it is indexed in Index Copernicus.

10 November 2017 4,699 6 View

What is the best method for Enhancing Gene Expression?

What is best method for enhancing the expression of a gene if you need a protein, is it increasing copy no or removing control from it or anything else?

10 November 2017 7,263 5 View

What is the difference between normal primers and primers for cloning?

I need to work on expression of a gene. can anyone tell me about the difference of these primers?

09 October 2017 6,390 4 View

How quickly the PCR primer deteriorates?

I used to use PCR primers even after 3-4 months and they worked, but now something seems to be wrong with them, they stop working after few runs. I am totally clueless about it.

09 October 2017 1,404 6 View

My PCR band disappeared after optimization, what should I do?

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09 October 2017 6,435 8 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

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01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

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01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

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26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

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Cloudy/high background western blot - can anyone help me?

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How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

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21 February 2021 5,379 2 View

How to improve the buffer capacity of sea water?

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Dy Dong

You'd better use the corresponding buffer in case of unknown circumstances.

Maxime Pichon

In case of commercial assays, I would recommend to use appropriate buffer to ensure optimal performances. An other RT buffer could also be used I think but performances might be lower ...

Nadia Jamil

Thank you Maxime Pichon and Deyuan Dong.