What is the proper method for cleaning of combs for agarose gel electrophoresis?

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Pietro Pilo Boyl

Hi Nadia,

better not boil, the aerosol might carry around the Ethidium Bromide.

Instead, Ethidium Bromide is inactivated with soda, just soak the combs in 1 M NaOH for 15-30 min. Then wash thoroughly in water. Careful with soda! Use gloves, don't splash it on your skin!

Paul Rutland

I agree boiling is not good and will distort the plastic combs. Just rinse it .Any ETBR staining the comb will not interfere with your gel running. Dilute acid or bleach will also remove EtBr stains

Nadia Jamil

Thank you Pietro Pilo Boyl and Paul Rutland for your kind answers. Really appreciate it. Pietro Pilo Boyl I will wash it with 1 M NaOH for 15-30 min.