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Questions related from Alemu Tekewe
We often induce protein expression when OD600 of E. coli culture reaches 0.4-0.8. What is/are the effect(s) of the pre-induction OD600 on expression level and stability of target proteins from...
03 March 2018 814 2 View
Co-expression of chaperones with recombinant proteins often enhances expression of soluble proteins. However, separation of some co-expressed chaperones from target proteins sometimes becomes a...
09 September 2017 1,084 3 View
I have purified a plasmid DNA using a plasmid miniprep kit for transforming into a bacterial expression system. I run an agarose gel for detection of the purified vector. I could detect three...
08 August 2017 3,314 4 View
Recently, I have performed PCR reactions to amplify different gene fragments for cloning. I usually included negative controls in my experiments. The negative controls contain all the reaction...
08 August 2017 2,500 10 View
I am trying to express toxic peptides using a bacterial expression system. Can someone share me an experience/ or any possible strategies for successful production of toxic peptides in bacteria?
08 August 2017 419 4 View
E. coli strains are good working horses for expressions of recombinant proteins. They have got huge potential for production of low-cost subunit vaccine candidates against various target diseases....
06 June 2017 2,342 12 View
Starch is the second most abundant polymer with broad applications. Is there any one with experience in producing starch in bacteria or yeasts using biotechnology or synthetic biology approaches?
03 March 2017 8,010 2 View
