Error while running the cif2cell script?

I am getting this error every time I run the cif2cell script, How can I solve the problem? $ cif2cell -f graphenerelaxed.cif -p quantum-espresso Traceback (most recent call last): File...

13 July 2020 6,425 1 View

How to install ShengBTE in ubuntu?

I am trying to install this software but it doesn't work

12 June 2020 6,352 1 View

Will it be sufficient to calculate properties of a material (in quantum espresso) using the unit cell only?

Will it be sufficient to calculate the properties of a material with the atoms in the unit cell only, like in the attached, or do I have to make a super-cell? what difference does it have?

22 May 2020 7,087 13 View

How can I calculated transport properties of layered materials across plane and in-plane?

I want to calculate the cross-plane and in-plane transport properties of layered materials using quantum espresso and boltztrap codes. I don't understand how to choose between in-plane and...

15 October 2019 5,573 2 View

How much does bead beating for cell lysis heat the samples (reg. pre-cooled adapters for the Qiagen Tissue Lyser II) ?

Hello, I would like to isolate total protein for phosphoproteomics using the Qiagen Tissue Lyser II (that we have in our department) for disruption of yeast lysis with glass beads. However,...

10 October 2019 8,861 3 View

How to prevent reoxygenation of cells after incubation in hypoxic chanbers?

We are using hypoxia chambers to induce physiological hypoxia in cell cultural environment. the major problem is, reoxygenation during the cell harvesting, cell lysis, etc. this is the chamber we...

08 June 2016 1,646 4 View

How to do the COMET ASSAY for cell line?

Complete PROTOCOL needed

18 April 2016 7,269 3 View

Does anybody know any hepatocellular carcinoma cancer cell line control drug for treatment?

27 September 2015 2,148 9 View

Is anyone familiar with performing MTT assay for Plant extracts?

MTT assay concentration fixing stock solution perpetration and require volume for test.

04 March 2015 150 2 View

Suppose I have an AC current source and I want to combine them to give maximum sum, should I use series or parallel combination?

How is the sum of current calculated?

29 January 2015 8,738 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View