Can any one suggest Protocol for isolation of proteins from cancer cell lines?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Matlab codes on Regular shape channel modelling?

i am working on regular shape channel modelling, to be precise one ring, two ring and ellipse stochastic channel modelling. Kindly assist with Matlab codes to enable me understand the topic more.

21 February 2024 191 0 View

What is your perception on the existence of "Religion" in the Third World Countries?

Religion is making people to become poorer everyday

09 January 2024 281 2 View

Which scholarly authorities support full mediation over partial mediation in structural equation modeling ?

I want current scholarly authorities which states that in the event of indirect effect ( mediation ) , the type of mediation should be ignored or not relevant in analysis. ( ie as it were in the...

18 July 2023 8,787 2 View

What are the differences in processes in Eviews Chow - Lin Data temporal Disaggregation and other data disaggregation methods in eviews ?

What are the processes of Eviews Chow-Lin temporal data disaggregation ?

08 May 2023 3,322 2 View

How do we calculate bandstructure using hybrid functionals in VASP?

How do we calculate bandstructure using hybrid functionals in VASP? I am trying to calculate band structure in VASP using the HSE06 hybrid functional but I get this error. In my calculation, I...

24 November 2022 6,895 7 View

How do we calculate bandstructure using hybrid functionals in quantum espresso?

I am trying to calculate band structure in QE using the HSE hybrid functional but I get a band structure that looks funny. I have attached the script I am using here(qeBands.sh) and the band...

24 November 2022 179 2 View

How do I handle four latent variables in SEM?

How do I handle four latent variables in SEM? Out of the four I have one to be a mediator, one to be a moderator, one as a dependent variable, and lastly one to be an independent variable. It's a...

14 December 2021 8,685 3 View

How Structural Equation Model fit in a relationship research study?

How would Structural Equation Model( SEM) methodology and data analysis fit in the study that seeks to establish the relationship between human development and five factor inflows? Any suggestion...

19 October 2021 7,789 3 View

What are the Filipino teachers’ experiences in teaching action research as embedded in the current teacher education curriculum?

1. How would you describe your overall experience in teaching action research? 2. What are the challenges that you have encountered in teaching the course? 3. Do you have suggestions on how the...

08 March 2021 7,709 10 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View