Drug inhibits protein, but the specific gene also get no or lower expression after 24hrs of incubation ?

An enzymatic functional protein has been targeted using small molecule compound. We succesfuly found that the compound has inhibited the growth of the organism. But, we found mRNA for that...

03 April 2018 818 4 View

Journal Citation download for MS office word ? Google scholar not working ?

HI all, This is a basic clarification, where I generally copy citation from Google scholar (typing title of article, and search to download the citation with API and other formats available to...

03 April 2018 6,706 2 View

CRISPR system in Pseudomonas ? acquired or Innate ?

Recently I found there are many CRISPR genes among my Pseudomonas aeruginosa genome study. My query is whether these could be adapted from any viral or other microbes or it is innate in some of...

07 August 2016 8,751 5 View

Efflux pump detection by PCR targeting MexAB-oprM like genes is really useful or QPCR based expression of efflux gene is necessary?

Dear researchers, I have identified the presence of efflux pump genes in only 65% of Pseudomonas and absent in 35% of Pseudomonas strains. Where detection of the gene by PCR targeting MexAB-oprM...

10 November 2015 911 1 View

How can I confirm the presence or absence of a protein after drug treatment using Western blot?

As per my research, drives me to inhibit a particular gene which codes for a protein involves in multiple functions. After treating the cells with my designed drug against that protein, i have...

05 June 2015 9,716 7 View

Dear Scientists, I would like to inhibit a growth or particular gene of bacteria (Ex. E.coli) using Small interferring RNA ?

Dear Scientists, I would like to inhibit a growth or particular gene of bacteria (Ex. E.coli or P aeruginosa ) using Small interferring RNA . Can you suggest me a good protocol - related...

05 June 2015 5,767 0 View

Suggest me a journal with above 3 impact factor- Free/or below 700 Usd publication for NGS based study?

Suggest me a journal with above 3 impact factor- Free/or below 700 Usd publication for Microbial genome sequencing -NGS based study? Especially Review period with in short duration (1-2...

01 February 2015 904 2 View

Can anyone suggest a protocol and recipient strain for conjugation experiment?

Dear scientists, I have an enormous number of pseudomonas aeruginosa drug resistant strains, I would like to analyse the gene transfer ability of those bacteria through conjugation experiment. As...

31 December 2014 6,067 10 View

Integron and its explanation in detail needed, what is ISKpn4a in integron cassette?

11 December 2014 6,638 1 View

Can you suggest me a title or research query for a person to get PhD in computer science/ Bioinformatics?

Dear Researchers, One of My friend completed M.Sc., M.Phil., Computer science, He wants to do Ph.D in Computer science, he is skilled in scripting and other computer languages, hence, I suggested...

11 December 2014 6,622 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View