Drug inhibits protein, but the specific gene also get no or lower expression after 24hrs of incubation ?

An enzymatic functional protein has been targeted using small molecule compound. We succesfuly found that the compound has inhibited the growth of the organism. But, we found mRNA for that...

03 April 2018 818 4 View

Journal Citation download for MS office word ? Google scholar not working ?

HI all, This is a basic clarification, where I generally copy citation from Google scholar (typing title of article, and search to download the citation with API and other formats available to...

03 April 2018 6,706 2 View

Can any one explain about Ampure beads ?

During NGS library preparation or any Bead based DNA washes carried out to remove below threshold size DNA based on the concentration of Ampure XP beads used in the respective steps. Still I...

11 December 2017 3,552 0 View

CRISPR system in Pseudomonas ? acquired or Innate ?

Recently I found there are many CRISPR genes among my Pseudomonas aeruginosa genome study. My query is whether these could be adapted from any viral or other microbes or it is innate in some of...

07 August 2016 8,751 5 View

Efflux pump detection by PCR targeting MexAB-oprM like genes is really useful or QPCR based expression of efflux gene is necessary?

Dear researchers, I have identified the presence of efflux pump genes in only 65% of Pseudomonas and absent in 35% of Pseudomonas strains. Where detection of the gene by PCR targeting MexAB-oprM...

10 November 2015 911 1 View

How can I confirm the presence or absence of a protein after drug treatment using Western blot?

As per my research, drives me to inhibit a particular gene which codes for a protein involves in multiple functions. After treating the cells with my designed drug against that protein, i have...

05 June 2015 9,716 7 View

Suggest me a journal with above 3 impact factor- Free/or below 700 Usd publication for NGS based study?

Suggest me a journal with above 3 impact factor- Free/or below 700 Usd publication for Microbial genome sequencing -NGS based study? Especially Review period with in short duration (1-2...

01 February 2015 904 2 View

Can anyone suggest a protocol and recipient strain for conjugation experiment?

Dear scientists, I have an enormous number of pseudomonas aeruginosa drug resistant strains, I would like to analyse the gene transfer ability of those bacteria through conjugation experiment. As...

31 December 2014 6,067 10 View

Integron and its explanation in detail needed, what is ISKpn4a in integron cassette?

11 December 2014 6,638 1 View

Can you suggest me a title or research query for a person to get PhD in computer science/ Bioinformatics?

Dear Researchers, One of My friend completed M.Sc., M.Phil., Computer science, He wants to do Ph.D in Computer science, he is skilled in scripting and other computer languages, hence, I suggested...

11 December 2014 6,622 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View