Drug inhibits protein, but the specific gene also get no or lower expression after 24hrs of incubation ?

An enzymatic functional protein has been targeted using small molecule compound. We succesfuly found that the compound has inhibited the growth of the organism. But, we found mRNA for that...

03 April 2018 818 4 View

Journal Citation download for MS office word ? Google scholar not working ?

HI all, This is a basic clarification, where I generally copy citation from Google scholar (typing title of article, and search to download the citation with API and other formats available to...

03 April 2018 6,706 2 View

Can any one explain about Ampure beads ?

During NGS library preparation or any Bead based DNA washes carried out to remove below threshold size DNA based on the concentration of Ampure XP beads used in the respective steps. Still I...

11 December 2017 3,552 0 View

Efflux pump detection by PCR targeting MexAB-oprM like genes is really useful or QPCR based expression of efflux gene is necessary?

Dear researchers, I have identified the presence of efflux pump genes in only 65% of Pseudomonas and absent in 35% of Pseudomonas strains. Where detection of the gene by PCR targeting MexAB-oprM...

10 November 2015 911 1 View

How can I confirm the presence or absence of a protein after drug treatment using Western blot?

As per my research, drives me to inhibit a particular gene which codes for a protein involves in multiple functions. After treating the cells with my designed drug against that protein, i have...

05 June 2015 9,716 7 View

Dear Scientists, I would like to inhibit a growth or particular gene of bacteria (Ex. E.coli) using Small interferring RNA ?

Dear Scientists, I would like to inhibit a growth or particular gene of bacteria (Ex. E.coli or P aeruginosa ) using Small interferring RNA . Can you suggest me a good protocol - related...

05 June 2015 5,767 0 View

Suggest me a journal with above 3 impact factor- Free/or below 700 Usd publication for NGS based study?

Suggest me a journal with above 3 impact factor- Free/or below 700 Usd publication for Microbial genome sequencing -NGS based study? Especially Review period with in short duration (1-2...

01 February 2015 904 2 View

Can anyone suggest a protocol and recipient strain for conjugation experiment?

Dear scientists, I have an enormous number of pseudomonas aeruginosa drug resistant strains, I would like to analyse the gene transfer ability of those bacteria through conjugation experiment. As...

31 December 2014 6,067 10 View

Integron and its explanation in detail needed, what is ISKpn4a in integron cassette?

11 December 2014 6,638 1 View

Can you suggest me a title or research query for a person to get PhD in computer science/ Bioinformatics?

Dear Researchers, One of My friend completed M.Sc., M.Phil., Computer science, He wants to do Ph.D in Computer science, he is skilled in scripting and other computer languages, hence, I suggested...

11 December 2014 6,622 2 View

No title

02 March 2021 3,060 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View