Drug inhibits protein, but the specific gene also get no or lower expression after 24hrs of incubation ?

An enzymatic functional protein has been targeted using small molecule compound. We succesfuly found that the compound has inhibited the growth of the organism. But, we found mRNA for that...

03 April 2018 818 4 View

Journal Citation download for MS office word ? Google scholar not working ?

HI all, This is a basic clarification, where I generally copy citation from Google scholar (typing title of article, and search to download the citation with API and other formats available to...

03 April 2018 6,706 2 View

Can any one explain about Ampure beads ?

During NGS library preparation or any Bead based DNA washes carried out to remove below threshold size DNA based on the concentration of Ampure XP beads used in the respective steps. Still I...

11 December 2017 3,552 0 View

CRISPR system in Pseudomonas ? acquired or Innate ?

Recently I found there are many CRISPR genes among my Pseudomonas aeruginosa genome study. My query is whether these could be adapted from any viral or other microbes or it is innate in some of...

07 August 2016 8,751 5 View

Efflux pump detection by PCR targeting MexAB-oprM like genes is really useful or QPCR based expression of efflux gene is necessary?

Dear researchers, I have identified the presence of efflux pump genes in only 65% of Pseudomonas and absent in 35% of Pseudomonas strains. Where detection of the gene by PCR targeting MexAB-oprM...

10 November 2015 911 1 View

How can I confirm the presence or absence of a protein after drug treatment using Western blot?

As per my research, drives me to inhibit a particular gene which codes for a protein involves in multiple functions. After treating the cells with my designed drug against that protein, i have...

05 June 2015 9,716 7 View

Dear Scientists, I would like to inhibit a growth or particular gene of bacteria (Ex. E.coli) using Small interferring RNA ?

Dear Scientists, I would like to inhibit a growth or particular gene of bacteria (Ex. E.coli or P aeruginosa ) using Small interferring RNA . Can you suggest me a good protocol - related...

05 June 2015 5,767 0 View

Can anyone suggest a protocol and recipient strain for conjugation experiment?

Dear scientists, I have an enormous number of pseudomonas aeruginosa drug resistant strains, I would like to analyse the gene transfer ability of those bacteria through conjugation experiment. As...

31 December 2014 6,067 10 View

Integron and its explanation in detail needed, what is ISKpn4a in integron cassette?

11 December 2014 6,638 1 View

Can you suggest me a title or research query for a person to get PhD in computer science/ Bioinformatics?

Dear Researchers, One of My friend completed M.Sc., M.Phil., Computer science, He wants to do Ph.D in Computer science, he is skilled in scripting and other computer languages, hence, I suggested...

11 December 2014 6,622 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View