02 February 2013 13 2K Report

I am facing some difficulties with caesin zymogram assaying the activity of MMP-3 and -7 from human samples. I am not getting any activity, but the proteins are there in the western blots. After running, some protein clumps are seen near the wells. I also tried the same with altering the SDS percentage, but it didn't work. Can anybody suggest a way out?

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