Ruchi Kakar

7 Questions 41 Answers 0 Followers

Questions related from Ruchi Kakar

Seeing lower molecular weight gelatinases in my sample?

I am seeing high gelatinase activity at site between 55 and 35 kDa and unable to find out the reason for this? Samples are MDA-MB-231 cell lysates and spent media. Lane 1: protein ladder Lanes 2...

03 January 2024 9,258 6 View

Time for E.coli to reach lysosomes?

I am planning to carry out gentamicin protection assay to study the internalization of E.coli in macrophages and dendritic cells. I want to specifically look at the degradation of E.coli in...

14 November 2021 9,510 2 View

Phagocytosis assay using E.coli GFP?

I need to carry out phagocytosis assay in RAW 264.7 cells using E.coli GFP for microscopic visualization. 1. How do I calculate bacterial number for the assay that can help me determine the MOI....

14 October 2021 8,718 8 View

Protein not being detected in western blot but seen in immunofluorescence and RNA confirmed by Real-Time PCR.

Rab11, a trafficking molecule does not come up as clear bands in western blotting but shows a lot of non specific bands. Same antibody when used for immunofluorescence shows intense staining. It...

05 February 2013 6,875 8 View

Cells become round and detached after changing to fresh medium in plate

I have been trying to transfect an endometrial epithelial cell line with a plasmid. But before transfection, when I change the medium of the cells and replenish with the same growth medium in...

21 January 2013 5,364 68 View

Can anybody suggest a good lysis buffer for Rab11A protein?

I know the question is very specific but this protein even being cytoplasmic, just does not come in any lysis buffer I have tried.

09 May 2012 7,379 4 View

Peptide increasing protein-protein interaction instead of inhibiting

Has anyone ever seen an increase in protein-protein interaction after using a peptide, which was ideally supposed to block or reduce interaction? The peptide mimics one protein's active site, so...

01 January 1970 3,586 5 View